Fluorescence Imaging: The First Demonstration of Far-Field, Subdiffraction Fluorescence Lifetime Imaging

Mar 11, 2013
By Spectroscopy Editors

An interview with Emily Smith, a finalist for the 2012 FACSS-SciX Innovation Award.

Part of the 2013 podcast series presented in collaboration with the Federation of Analytical Chemistry and Spectroscopy Societies (FACSS), in connection with SciX 2013, the federation’s North American conference.

For more podcasts in this series from 2013 and 2012, visit http://www.spectroscopyonline.com/podcasts

Emily Smith is an assistant professor in the Department of Chemistry at Iowa State University in Ames, Iowa.

She was a finalist for the 2012 FACSS Innovation Award, for the following paper:

“Subdiffraction Fluorescence Lifetime Imaging” (Emily A. Smith, Michael Lesoine, Aleem Syed, Sayantan Bose, and Jacob Petrich).

Professor Smith and her coworkers developed a stimulated emission depletion (STED) fluorescence microscope and used it to collect fluorescence lifetime images. With this approach, they were able to achieve a lateral spatial resolution of 40 nm, which is an eightfold improvement in lateral resolution compared with traditional far-field optical microscopy. This system has been used to measure the organization of integrin cell membrane proteins and the cellular factors that affect integrin rearrangement.

About the FACSS Innovation Award

The FACSS Innovation Award showcases the newest and most creative science debuted orally at a FACSS-organized conference.

More about the SciX conference:

The North American meeting of FACSS is now called “SciX — The Great Scientific Exchange.” SciX covers the whole of analytical chemistry with an emphasis on emerging technologies. SciX carries the FACSS tradition of a strong technical program (over 100 symposia), many prestigious awards, exhibits, a variety of workshops, an employment bureau and multiple social networking opportunities.

SciX is the National Meeting of the Society for Applied Spectroscopy (SAS) and the North American Society for Laser-Induced Breakdown Spectroscopy (NASLIBS).

For more information about the SciX 2013 conference in Milwaukee, Wisconsin, September 29 to October 4, 2013, visit scixconference.org

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