Articles by Waters Corporation - Chromatography Online
Articles by Waters Corporation

Waters EU - High-Resolution Peptide Mapping Separations with MS-Friendly Mobile Phases and Charge-Surface-Modified C18

Jun 23, 2014

This work demonstrates that a novel charged-surface C18 (CSH C18) stationary phase alleviates some of the challenges associated with reverse-phase peptide separations. CSH C18 columns improve loadability, increase peak capacities, and offer unique selectivity compared to conventional C18 columns. . Performance is significantly less dependent on TFA-ion pairing, making it ideal for MS applications where high sensitivity is desired.

Waters EU - An Introduction to the Capabilities of Microscale 2D-RP-RP Peptide Chromatography with an ACQUITY UPLC M-Class System

Jun 23, 2014

This technology brief demonstrates that 2D-RP-RP chromatography operated at ≥10 K psi with an ACQUITY UPLC M-Class and an ACQUITY UPLC M-Class 300 μm analytical column can be a robust solution for obtaining high peak capacity. It will be shown that such a system affords high resolving power and outstanding chromatographic reproducibility and performance with extended use.

Waters EU - Future-Proofing the Biopharmaceutical QC Laboratory: Integrating AutoBlend Technology to Improve Peptide Mapping

Jun 23, 2014

This application note demonstrates the ability of Waters Auto•Blend Technology to control the trifluoroacetic acid (TFA) component of the mobile phase during routine peptide mapping analyses, thereby producing chromatograms of equivalent performance to HPLC-acquired chromatograms with conventionally modified mobile phases.

Waters EU - High Mass Loading of Peptides with Hybrid Particle C18 Columns and Acetic Acid Mobile Phases

Jun 23, 2014

This study investigates the use of both BEH C18 and CSH? C18 Columns for preparative peptide separations. The presented data demonstrate that both BEH C18 and CSH C18 Columns can yield narrower target peaks at high mass loads with optimized acetic acid mobile phases than those containing 0.1% TFA.

Waters EU - Increasing Peak Capacity in Reversed-Phase Peptide Separations with Charged Surface Hybrid (CSH) C18 Columns

Jun 23, 2014

In this study, we evaluate charged surface hybrid (CSH) C18 column chemistry for peptide separations, expecting similar improvements in chromatographic performance since most peptides under commonly employed acidic conditions contain a positive charge. This work with peptides demonstrates that this novel stationary phase exhibits greater peak capacity, unique selectivity, and less dependence on MS signal suppression, strong ion pairing agents in peptide analysis.

Waters NA - Peptide Mapping and Small Protein Separations with Charged Surface Hybrid (CSH) C18 and TFA-Free Mobile Phases

Jun 20, 2014

In this study, the use of CSH130 C18 Column with FA mobile phases is further investigated with the analysis of more demanding separations. LC-MS of an enolase tryptic digest is compared among a CSH130 C18, BEH130 C18, and a superficially porous C18 column. In addition, the applicability of these columns for separations of polypeptides up to 12 kDa is evaluated.

Waters NA - High Mass Loading of Peptides with Hybrid Particle C18 Columns and Acetic Acid Mobile Phases

Jun 20, 2014

This study investigates the use of both BEH C18 and CSH? C18 Columns for preparative peptide separations. The presented data demonstrate that both BEH C18 and CSH C18 Columns can yield narrower target peaks at high mass loads with optimized acetic acid mobile phases than those containing 0.1% TFA.

Waters NA - Charged Surface Hybrid C18 for High Peak Capacity nanoLC Peptide Separations

Jun 20, 2014

We demonstrate the unique ability of charged surface hybrid (CSH™ Technology) C18 to produce high peak capacity peptide separations at high mass loads in nanoLC chromatography.

Waters NA - An Introduction to the Capabilities of Microscale 2D-RP/RP Peptide Chromatography with an ACQUITY UPLC M-Class System

Jun 20, 2014

This technology brief demonstrates that 2D-RP/RP chromatography operated at ?10K psi with an ACQUITY UPLC M-Class and an ACQUITY UPLC M-Class 300 ?m analytical column can be a robust solution for obtaining high peak capacity. It will be shown that such a system affords high resolving power and outstanding chromatographic reproducibility and performance with extended use

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LCGC COLUMNISTS 2014

Column Watch | Ronald E. Majors: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: Standardized Testing of Silica as a Base Material for Difficult Bonded-Phase Preparative Applications


Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: Seven Common Faux Pas in Modern HPLC


MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: How Much Retention Time Variation Is Normal?


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