Mass Spectrometry

Jul 01, 2017
Special Issues
There is growing interest in the determination of endogenous proteins in biological samples for diagnostic purposes, because a concentration increase or decrease of such proteins can allows us to monitor the state of a pathological condition such as cancer. Immunocapture LC–MS/MS analysis combines the workflow of conventional immunological assays with LC–MS analysis. This article describes typical challenges, such as cross reactivity and the mass spectrometer’s dynamic range, as well as the advantages of isoform differentiation and multiplexing.
Jul 01, 2017
Special Issues
In this study, general extract screening of food storage materials was done with nontargeted analytical methods to understand what analytes could potentially leach into food or beverages. GC and mass spectral deconvolution effectively separated analytes within the complex mixture and TOF-MS provided full mass range spectral data for identification. This workflow can be used for confident characterization of components present as extractables from food packaging materials.
May 01, 2017
Special Issues
Simultaneous, enantiomer-specific identification of chiral molecules in multicomponent mixtures is extremely challenging. With mass-selected photoelectron circular dichroism (MS-PECD) using an electron–ion coincidence imaging spectrometer, a compound can be identified as chiral without the need for any prior enantiomeric separation or enantiomer-selective complexation.
May 01, 2017
Special Issues
The analysis of oil samples containing many thousands of constituents best illustrates the benefits of ion mobility MS for complex samples. Here, we test the limits of ion mobility MS to discern differences between batches of Copaxone, a highly complex drug containing billions of peptides, and various purported generic versions of the drug.
May 01, 2017
Spectroscopy
This study demonstrates a new imaging approach by coupling LA with ICP-TOF-MS in two application areas: geology and biology.
May 01, 2017
Special Issues
The analysis of oil samples containing many thousands of constituents best illustrates the benefits of ion mobility MS for complex samples. Here, we test the limits of ion mobility MS to discern differences between batches of Copaxone, a highly complex drug containing billions of peptides, and various purported generic versions of the drug.
May 01, 2017
Special Issues
With computational chemistry, chemists can now study chemical phenomena by performing computationally intense calculations on computers rather than examining reactions and compounds experimentally. This is especially attractive when the laboratory experiments are time consuming, costly, dangerous, or difficult. Modern computational chemistry tools are capable of determining molecular structures, molecular spectra, and energetics, and of elucidating reaction pathways and chemical reaction products.
May 01, 2017
Special Issues
In this study, a simple method was used for extraction and concentration of trace organic compounds in water, followed by injection using a coiled wire filament and GC–MS analysis. Common semivolatile organic compound contaminants at low parts-per-billion levels were detected in less than 10 min.
May 01, 2017
Special Issues
Simultaneous, enantiomer-specific identification of chiral molecules in multicomponent mixtures is extremely challenging. With mass-selected photoelectron circular dichroism (MS-PECD) using an electron–ion coincidence imaging spectrometer, a compound can be identified as chiral without the need for any prior enantiomeric separation or enantiomer-selective complexation.
Mar 01, 2017
Special Issues
A new peptide mapping method was developed specifically for mAb characterization that employs optimal enzyme pH for robustness, but with short digestion times and time-course elements to minimize and monitor deamidation–isomerization, respectively, enabling a more accurate assessment of potential CDR sequence liabilities.
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