Chlorophyll and leaf water content are important nutritional indicators of the Gannan navel orange tree. Hyperspectral imaging was used to simultaneously estimate chlorophyll and water content of Gannan navel orange leaves. The genetic algorithm (GA) and successive projections algorithm (SPA) were applied in selecting characteristic wavelengths after average spectra were extracted from the region of interest (ROI). The hyperspectral effective information was extracted using the partial least squares (PLS) model. The results derived from these techniques were then compared. The prediction model of chlorophyll and water content built using SPA on 8 and 14 selected wavelengths (3.15% and 5.15% of the total number of wavelengths) exhibited higher coefficients of determination r of 0.98 and 0.93 and root mean square error of prediction (RMSEP) values of 3.40 and 0.04, respectively. The results show that the accuracy of the quantitative analysis conducted by hyperspectral imaging can be improved through appropriate wavelength selection. Although the RMSEP value of SPA-PLS was slightly higher than that of GA-PLS, the SPA-PLS model can be used for applications because it was simpler and easier to interpret.
Chlorophyll and leaf water content indirectly reflect the ability of plant growth and photosynthesis and they are closely related to the nutritional status of the plant, which is one of the most important indicators of the Gannan navel orange photosynthetic capacity and developmental stage. Chlorophyll is a green pigment found in cyan bacteria and the chloroplasts of algae and plants. It is an extremely important biomolecule that is critical in photosynthesis, which allows plants to absorb energy from light. Chlorophyll absorbs light mostly in the blue region of the electromagnetic spectrum. However, chlorophyll is a poor absorber of green and near-green regions of the spectrum, hence the green color of tissues containing chlorophyll (1). Traditional measurement of chlorophyll and water in a leaf damages the blade and is time consuming. Typical methods include spectrophotometry (2) and gravimetric methods.
In recent years, hyperspectral imaging has gained wide acceptance in different fields by virtue of its advantages over other analytical techniques. The most important advantage is its ability to record spectra and images simultaneously. The rapid, nondestructive, and reliable features were first used in military and remote sensing (3), and then were applied in astronomy (4), food (5), agriculture (6), and pharmaceuticals (7). Hyperspectral imaging at visible (vis) and short near infrared (SNIR) regions has been used to estimate chlorophyll or water content (8–10) in leaf samples.Among numerous wavelength selection methods, partial least squares (PLS) has been extensively used to enhance the performance of models constructed (11). Genetic algorithms (GA) add complexity to the computation when the number of selected wavelengths increases (12). Sorol and colleagues (13) applied several wavelength selection algorithms to sort informative wavelengths in building a PLS model to predict the degrees Brix (sugar content) of sugar cane juice. They found that the best results were obtained using GA. A novel forward selection method called the successive projections algorithm (SPA) has been used to obtain a simpler model for minimizing wavelength collinearity. Chen and colleagues (14) used SPA to optimize the NIR spectroscopic quantitative model for potable spirits; they obtained a good predictive correlative coefficient of 0.9477 using only 1.17% of the total number of wavelengths.
Materials and Methods
where W is water content, W 1 is leaf fresh weight, and W 2 is leaf dry weight.
where I λ is the recorded hyperspectral image, B λ is the dark image (with 0% reflectance) recorded by turning off the lighting source with the lens of the camera completely closed, and W λ is the white reference image (PTFE white board with 99% reflectance) (16). The above data collection and correction processes were implemented using software called spectraSENS1.5.