Jerome Workman, Jr. serves on the Editorial Advisory Board of Spectroscopy and is currently with Unity Scientific LLC. He is also an adjunct professor at Liberty University and U.S. National University. He can be reached at firstname.lastname@example.org.
Now that we have shown the relationships between different units for concentration, we continue by demonstrating their effects on the data we collected and used for our examples. What are the ramifications and consequences of these findings?
What are the techniques and mathematics used to compute uncertainty, and the optimum methods for maintaining wavelength accuracy within instrumentation over time, when considering measurement condition changes?
The data show that different units of measurement have different relationships to the spectral values, for reasons having nothing to do with the spectroscopy. This finding disproves the assumption that different measures of concentration are equivalent except, perhaps, for a constant scaling factor.
The statistical methods used for evaluating the agreement between two or more instruments (or methods) for reported analytical results are discussed, with an emphasis on acceptable analytical accuracy and confidence levels using two standard approaches, standard uncertainty or relative standard uncertainty, and Bland-Altman "limits of agreement."
Calibration transfer is a series of techniques used to apply a single spectral database, and the calibration model developed using that database, to two or more instruments. Here, we review the mathematical approaches and issues related to the calibration transfer process.