Ep. 20: Using multi-dimensional gas chromatography to help people breathe easier


In this episode, podcast co-hosts Dr. Dwight Stoll and Dr. James Grinias talk with Dr. Heather Bean, Associate Professor of Biomedicine and Biotechnology at Arizona State University. Dr. Bean is an expert in volatilomics and leads a research program focused on the use of omics approaches to better understand a variety of physiological conditions ranging from disease and illness, such as cystic fibrosis and Valley fever, to fatigue. In their conversation, they discuss several of Dr. Bean’s recent research articles focused on the use of two-dimensional gas chromatography coupled with mass spectrometry to profile hundreds of volatile compounds present in biological samples that are relevant to these conditions. In addition to aspects of the actual separations that produce these data, they also discuss some of the current challenges in this type of work, including “missingness” in the datasets, and the importance of retention databases to omics work.

Listeners are also referred to the upcoming Multidimensional Chromatography Workshop, which will be held at California State University, Los Angeles, from January 10-12. For more information, see the conference website - http://www.multidimensionalchromatography.com/.

Literature Discussed:

  • Davis, T.J.; Firzli, T.R.; Higgins Keppler, E.A.; Richardson, M.; Bean, H.D., Addressing Missing Data in GC × GC Metabolomics: Identifying Missingness Type and Evaluating the Impact of Imputation Methods on Experimental Replication, Analytical Chemistry 94, 2022, 10912–10920. https://doi.org/10.1021/acs.analchem.1c04093.
  • Higgins Keppler, E.A.; Mead, H.L.; Barker, B.M.; Bean, H.D., Life Cycle Dominates the Volatilome Character of Dimorphic Fungus Coccidioides spp., mSphere 6, 2021, e00040-21. https://doi.org/10.1128/mSphere.00040-21.
  • Hahn, A.; Whiteson, K.; Davis, T.J.; Phan, J.; Sami, I.; Koumbourlis, A.C.; Freishtat, R.J.; Crandall, K.A.; Bean, H.D., Longitudinal Associations of the Cystic Fibrosis Airway Microbiome and Volatile Metabolites: A Case Study, Frontiers in Cellular Infectious Microbiology 10, 2020, 174. https://doi.org/10.3389/fcimb.2020.00174.

More about our hosts:

Dwight Stoll, PhD:

Dwight R. Stoll is a professor of chemistry at Gustavus Adolphus College in St. Peter, Minnesota. He received his PhD from the University of Minnesota, under Professor Peter Carr, working on the development of fast, comprehensive two-dimensional liquid chromatography (2D-LC). Stoll’s current primary research focus is on the development of 2D-LC for both targeted and untargeted analyses. Active research projects in his laboratory touch on most aspects of multidimensional separation methodologies, including optimization strategies, characterization of selectivity in reversed-phase LC, instrument development, and applications in biopharmaceutical analysis. Stoll is the author or co-author of more than 80 peer-reviewed publications and six book chapters and has instructed numerous short courses in 2D-LC. In 2011 he was the recipient of LCGC’s Emerging Leader in Chromatography Award. In 2017 he received the Georges Guiochon Faculty Fellowship, and was recognized with an Agilent Technologies Thought Leader Award. He is also a member of LCGC’s editorial advisory board and is the editor of the “LC Troubleshooting” column in LCGC.

Jerome Workman, Jr., PhD:

Jerome (Jerry) J. Workman, Jr. is the Senior Technical Editor for LCGC and Spectroscopy. He has held positions as CTO, executive VP, senior research fellow, director, and senior scientist at companies of all sizes, from start-ups to world-leading corporations. He has been an adjunct faculty member of four universities and advised multiple graduate students. He has more than 75 U.S. and international patent applications and 30 issued U.S. and international patents and multiple trade secrets, as well as 500+ technical publications, and 20 reference book volumes on a broad range of spectroscopy and data processing techniques. He has received multiple awards from scientific societies, and has taught annual courses in spectroscopy, chemometrics, and statistics for the AOAC, ACS, ISA, FACSS, and at several universities and corporations. He is a Fellow of the American Institute of Chemists (FAIC), the American Society for Testing and Materials (ASTM), and the Royal Society of Chemistry in the UK (FRSC, CChem, CSci). Jerry holds B.A and M.A degrees from Saint Mary's University of Minnesota, and a PhD degree from Columbia Pacific University working in near-infrared spectroscopy. He is an alumnus of both Columbia University Business School and the MIT Sloan School of Management.

About the Analytically Speaking Podcast:

Analytically Speaking, the podcast from LCGC and Spectroscopy, addresses important issues in separation science and analytical spectroscopy. Topics include new analytical techniques, methods, and approaches; the latest trends; advances in instrument and software technology; practical solutions for specific applications; recent papers in the scientific literature and their applicability; challenges and solutions for data analysis and interpretation; analytical chemistry theory and fundamentals (from advanced research to tutorials and troubleshooting); and more. Our regular hosts are Dwight Stoll, PhD, a professor of chemistry at Gustavus Adolphus College in St. Peter, Minnesota, and Jerry Workman, PhD, a spectroscopist, noted author, and currently the Senior Technical Editor of Spectroscopy and LCGC. Dwight covers separation science and Jerry addresses spectroscopy related topics.

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