UV-vis Spectroscopy

Far-Ultraviolet Spectroscopy

June 01, 2020


In celebration of Spectroscopy’s 35th Anniversary, leading experts discuss important issues and challenges in analytical spectroscopy.

Review of New Spectroscopic Instrumentation 2019

May 01, 2019


Our annual review of products introduced at Pittcon or during the previous year

Overview of High-Efficiency Transmission Gratings for Molecular Spectroscopy

April 01, 2014


This article provides a basic overview of the capabilities of transmission gratings optimized for molecular spectroscopy.

Scattering Impact Analysis and Correction for Leaf Biochemical Parameter Estimation Using Vis–NIR Spectroscopy

July 01, 2011


Simulated leaf spectral data were generated to analyze scattering impact and then compared to experimental data to validate the conclusions of the simulation.

An Integration of Modified Uninformative Variable Elimination and Wavelet Packet Transform for Variable Selection

April 01, 2011


The wavelet packet transform (WPT) combined with the modified uninformative variable elimination (MUVE) method (WPT–MUVE) is proposed to select variables for multivariate calibration of spectral data.

Analysis of Fructose, Glycine, and Triglycine Using HPLC UV-vis Detection and Evaporative Light-Scattering Detection

August 11, 2009


The development of a method for the simultaneous determination of glycine, triglycine and fructose using UV–vis and evaporative light-scattering detection (ELSD) is described. This was necessary as part of a research project dealing with the recovery of functional peptides from aqueous streams on an industrial scale using adsorption or related technologies. Fructose is barely detectable by UV–vis as it lacks detectable functionalities, while glycine and triglycine are both UV–vis sensitive. An NH2 phase was chosen as a column and separation was obtained within seven minutes on a 250 X 4.6 mm column. Limits of detection are approximately 40 mg fructose/L, 4 mg glycine/L and 0.05 mg triglycine/L. Calibration functions are linear in a range of 40–1400 mg/L for fructose, 5–200 mg/L for glycine and 0.5–70 mg/L for triglycine.

Spectroscopy in Space: Hubble and the Cosmic Origins Spectrograph

May 15, 2009

In an upcoming spacewalk, shuttle astronauts will swap the Corrective Optics Space Telescope Axial Replacement (COSTAR) device for the new Cosmic Origins Spectrograph (COS).

UV-Vis Diffuse Reflectance of Thermochromic Materials Including Color-Changing Paint

February 01, 2009

Application Notebook

Some powders and rough surfaced solids change color as a function of temperature, a phenomenon known as thermochromism. Such phenomena can be effectively studied by UV-Visible spectroscopy in combination with a diffuse reflection accessory equipped with temperature-controlled reaction chamber, as demonstrated here with a thermal paint.

Liquid Matrices for Analyses by UV-MALDI Mass Spectrometry

August 07, 2008

Data are presented for a pH-adjustable liquid UV-matrix-assisted laser desorption ionization (MALDI) matrix for mass spectrometry analysis. The liquid matrix system possesses high analytical sensitivity within the same order of magnitude as that achievable by the commonly used solid UV-MALDI matrices but with improved spot homogeneity and reproducibility. The pH of the matrix has been adjusted, achieving an on-target pH range of 3.5?8.6, which has allowed for the performance of a tryptic digest within the diluted pH-optimized liquid matrix.

Ultraviolet Signals in Blue Tits

March 01, 2008

Application Notebook