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A new method based on singular value decomposition (SVD) applied to the
denoising of the time-resolved spectral matrix (TRSM) made it possible to
obtain kinetics data of the fluorescence band parameters of a
3-aminophthalimide spectrum in acetonitrile.

Smoothing of the Time-Resolved Spectral Matrix Obtained by a Streak Camera: Estimation of the Fluorescence Band Parameters

, is the General Manager of the Applied Systems business at Ocean Insight, an affiliate associate professor at the University of Washington, and has started and advised numerous companies in spectroscopy and in applications of machine learning. He has approximately 40 peer-reviewed publications and 6 patents. His work in practical optical spectroscopy, such as LIBS, Raman, and TDL spectroscopy, dovetails with the coverage in this column, which reviews methods (new and old) in laser-based spectroscopy and optical sensing.

Accurately measuring and quantifying the perception of color is an ongoing challenge for scientists, but understanding spectroscopic techniques can help standardize color measurements.

Where Perception Meets Reality: The Science of Measuring Color

A kinetic fluorimetric method was established for the determination of formaldehyde in synthetic water samples and laundry water by potassium bromate oxidation of rhodamine B.

Kinetic Fluorimetric Determination of Formaldehyde by Oxidation of Rhodamine B with Potassium Bromate

Xi Wang is with the State Key Laboratory Base of Novel Function Materials and Preparation Science at the School of Material Sciences and Chemical Engineering of Ningbo University, in Ningbo, Zhejiang, China.

Zongyue Chen is with the State Key Laboratory Base of Novel Function Materials and Preparation Science at the School of Material Sciences and Chemical Engineering of Ningbo University, in Ningbo, Zhejiang, China.

Jianyu Zhang is with the State Key Laboratory Base of Novel Function Materials and Preparation Science at the School of Material Sciences and Chemical Engineering of Ningbo University, in Ningbo, Zhejiang, China.

Shangke Pan is with the State Key Laboratory Base of Novel Function Materials and Preparation Science at the School of Material Sciences and Chemical Engineering of Ningbo University, in Ningbo, Zhejiang, China.

Jianguo Pan is with the Key Laboratory of Photoelectric Materials and Devices of Zhejiang Province, in Zhejiang, China. 

The possible energy transfer modes between Yb3+ and Tm3+ ions were analyzed.

Growth and Temperature-Dependent Spectral Properties of Yb3+, Tm3+ Co-Doped NaY(MoO4)2 Crystal

Xiao Long Sun is with the College of Materials and Textiles at Zhejiang SciTech University, in Zhejiang, China. 

Xiangshu Ma is with the College of Materials and Textiles at Zhejiang SciTech University, in Zhejiang, China. 

Yichuan Fang is with the College of Materials and Textiles at Zhejiang SciTech University, in Zhejiang, China. 

The relationship between the complexation amount of thorium (Th) and Suwannee River fulvic acid (SRFA) and the changes in Th concentration and pH were studied using differential spectroscopy and 3D excitation-emission matrix fluorescence spectroscopy (3D EEM).

Optical Properties of the Suwannee River Fulvic Acid Complexation with Thorium Using 3D Fluorescence Spectroscopy

Förster resonance energy transfer (FRET) is a versatile part of the toolbox of fluorescence methods. This through-space, photon-less energy transfer process between a donor fluorophore and an acceptor chromophore is perhaps most famous for its utility as a “molecular ruler” that can resolve nanometer-scale distances. FRET is also a popular and advantageous basis for biomolecular assays and sensors.

Key Steps to Follow in a FRET Experiment

A simple colorimetric and fluorescent dual-channel chemosensor was designed and synthesized to identify Hg2+ in an aqueous solution with demonstrated high selectivity and sensitivity.

A Novel High Selectivity Fluorimetric and Colorimetric Probe Based on Rhodamine B Hydrazide Derivatives for Detecting Hg2+ in Aqueous Media

Time-resolved fluorescence spectroscopy reveals much about the structure-induced energy transfer mechanisms of phycobilisomes, the light-harvesting antenna in cyanobacteria.

Time-resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisome of Thermophilic Cyanobacterium Thermosynechococcus vulcanus NIES 2134

Help for handling sample-specific concerns in spectrofluorometry.

Challenges of Spectrofluorometry Part 3: Sample-Specific Concerns

In the second part of a three-part series, instrument-specific concerns that require modifications to reproduce fluorescence spectra accurately are addressed.