Innovative Calibration Technique Enables Accurate Filter Fluorometer Readings


A new study introduces a filter fluorometer calibration method that eliminates the need for the fluorometer itself.

A new method that can calibrate filter fluorometers without relying on the device itself is possible, according to a study published in Applied Spectroscopy (1). The study, which was conducted by researchers at the University of South Carolina, used a technique that can estimate the calibration slope and detection limit for fluorescent analytes in water using system component performance and conventional spectrofluorometry (1). The researchers' work showcases the power of modeling techniques in accurately estimating calibration parameters (1).

Abstract flowing neon color wave lights background. Generative AI AIG21. | Image Credit: © Blue Planet Studio -

Abstract flowing neon color wave lights background. Generative AI AIG21. | Image Credit: © Blue Planet Studio -

The research team introduced a portable filter fluorometer that employed a 180° backscatter geometry for detecting chlorophyll-a (chl-a) in a lightweight, low-power, and waterproof design (1). All-encompassing modeling was performed to guarantee optimal performance. Building upon this prior work, the researchers repeated the modeling process in their recent study to estimate the calibration slope and detection limit (1).

Chlorophyll-a (chl-a) measurements are vital in plant analysis for multiple reasons. First, chl-a is the primary pigment responsible for photosynthesis, making it crucial for understanding a plant's ability to capture and utilize light energy (1). Second, chl-a serves as an indicator of plant health, allowing for the early detection of stress, nutrient deficiencies, or diseases (1). Third, chl-a measurements help study phytoplankton and algae populations in aquatic ecosystems, aiding in water quality assessment and the detection of harmful algal blooms (1). Fourth, chl-a plays a role in estimating primary productivity and carbon fixation rates, providing insights into ecosystem dynamics and global carbon budgets (1).

The research team achieved promising outcomes. By utilizing the model and comparing it to experimental calibration results from the fully assembled instrument, they realized the possibility of the new calibration approach. The model yielded a calibration slope of 8.22 mV-L/µg for dissolved chl-a, closely aligned with the experimentally measured slope of 8.21 mV-L/µg (1). Furthermore, the detection limit was estimated at 0.15 µg/L chl-a based on the slope and an estimated baseline noise of the instrument (1). The measured detection limit using real blank samples in 0.1-s differential measurements was 0.18 µg/L (1).

This new calibration approach eliminates the need for the actual filter fluorometer during the calibration process, offering potential time and cost savings in laboratory settings and field applications (1). The technique relies on comprehensive modeling and validation against experimental data, ensuring accuracy and reliability (1).

The successful demonstration of this calibration method opens up new possibilities for simplifying calibration procedures and enhancing the efficiency of filter fluorometry. Therefore, this study contributes to advancing the field and facilitating future advancements in filter fluorometer calibration and application.


(1) English, C. M.; Kitzhaber, Z. B.; Sanim, K. R. I.; Vitzilaios, N.; Hodgson, M. E.; Richardson, T. L.; Myrick, M. L. Filter Fluorometer Calibration Without the Fluorometer. Appl. Spectrosc. 2023, ASAP. DOI: 10.1177/00037028231181593

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