Monday Afternoon Session Preview: Advances in Ion Imaging

May 21, 2010

This session includes presentations of studies performed using mass spectrometry imaging techniques ranging from matrix-assisted laser desorption-ionization (MALDI) MS imaging to rapid evaporative ionization MS (REIMS) for applications such as 3D assessment of tumors, acquiring information about metabolic pathways of diabetes, and examination of metabolic exchange in bacteria.

Ballroom ACE

This session includes presentations of studies performed using mass spectrometry imaging techniques ranging from matrix-assisted laser desorption-ionization (MALDI) MS imaging to rapid evaporative ionization MS (REIMS) for applications such as 3D assessment of tumors, acquiring information about metabolic pathways of diabetes, and examination of metabolic exchange in bacteria.

The first presentation in the session will be given by Pierre Chaurand of the University of Montreal (Montreal, Canada) and is titled “Imaging Mass Spectrometry: Current Performance and Upcoming Challenges.” Chaurand will present an overview of the development of imaging MS and discuss the state of the art of the technology, including sample preparation, instrumentation, and data analysis.

The next talk, to be delivered by Erin H. Seeley of Vanderbilt University (Nashville, Tennessee) is titled “Correlation of MS, MRI, and Optical Images for 3D Assessment of the Tumor Microenvironment” and will discuss the use of the different techniques to examine the anti-tumor activity of an antibody in human breast cancer cells in immunocompromised mice.

Pieter Dorrestein of the University of California, San Diego, Skaggs School (La Jolla, California) will present the next talk, “Revealing Bacterial Post-Translationally Modified Cannibalistic Metabolic Exchange Factors with Imaging Mass Spectrometry.” Dorrestein’s presentation will discuss the use of imaging mass spectrometry of bacteria cultured on a MALDI plate.

The fourth presentation in the session, “More Information in Less Time: Strategies for High Spatial and High Mass Spectral Resolution Imaging Utilizing a Hybrid LIT-Orbitrap MS,” will be delivered by David C. Perdian of Iowa State University, Ames Laboratory US DOE (Ames, Iowa). The presentation will discuss imaging MS data acquisition strategies that scan both the linear ion trap and orbitrap mass analyzers in tandem to acquire information and minimize data acquisition time.

The penultimate presentation will be given by Raf Van de Plas of K.U. Leuven – SCD-SISTA Bioinformatics and K.U. Leuven – ProMeta Core Facility (Leuven, Belgium) and is titled “Going Beyond Images: Exploration of Hormone Processing Pathways in Diabetic Mouse Models via Mass Spectral Imaging and Data-Mining.” According to the authors, the data-mining method provides diabetes metabolic pathway screening directly from tissue and surpasses standard molecular imaging uses of MS imaging.

Finally, Zoltan Takats of the Justus-Liebig-University (Giessen, Germany) will present “Intraoperative Identification of Malignant Gastrointestinal Tumors and Proximal Metastases by Rapid Evaporative Ionization Mass Spectrometry.” This presentation will discuss the use of the technique to analyze ions formed during electrosurgical dissection in a surgical environment.