Unveiling the Healing Process: Fluorescence Properties of Wounds on Soybean Seedlings Explored

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A research team has developed a nondestructive method to assess the healing ability of plant tissues by analyzing the fluorescence properties of wounds on soybean seedlings.

A recent study published in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy explored plant wound healing by examining the fluorescence properties of wounds on soybean seedlings (1). Excitation emission matrix (EEM) and fluorescence imaging was used in this study (1). What the researchers discovered opens up further exploration of plant regeneration, which can have positive implications for the future of plant-based industries and sustainable agricultural practices (1).

Plants, like humans, are a living organism. Because of this fact, they can heal just like humans to. The researchers sought in this study to learn about plant regeneration processes. To achieve this, the team at Niigata University developed a simple and nondestructive method for evaluating the healing ability of plant tissues (1).

The procedure used is as follows: wounds were created on the stem of soybean seedlings. precisely seven days after sowing. Then, using fluorescence, the research team monitored the wounds over a period of 96 hours (1). By employing excitation emission matrix (EEM) and fluorescence imaging techniques, the researchers captured valuable data that shed light on the healing process at a microscopic level (1).

By using EEM to analyze the wounds, researchers detected the presence of three prominent fluorescence peaks, each exhibiting a decreasing intensity as the healing progressed (1). Moreover, the fluorescence images excited by a wavelength of 365 nm showcased a gradual reduction in the reddish color associated with chlorophyll, indicating a correlation between fluorescence properties and the healing process (1).


A confocal laser microscope was used to study the wounded tissue. This examination uncovered an intriguing phenomenon: the intensity of lignin or suberin-like fluorescence increased with healing time, suggesting that these substances might play a role in blocking the excitation light (1).

These findings open up new avenues for nondestructive assessment of plant wound healing (1). The ability to evaluate the healing process through fluorescence properties offers a valuable tool for plant biologists and agricultural scientists seeking to enhance crop health and productivity. By understanding the intricacies of plant regeneration and response to injuries, researchers can devise targeted strategies to promote healing and resilience in plants (1).

The team's work marks a significant step forward in our comprehension of plant wound healing. It sets the stage for future investigations and paves the way for the development of innovative techniques and tools that will contribute to more sustainable and productive agricultural practices.


(1) Saito, Y.; Ito, Y.; Tada, T.; Shoda, A.; Shiraiwa, T.; Kondo, N. Characterization of fluorescence properties of wounds on soybean seedlings during healing process using excitation emission matrix and fluorescence imaging. Spectrochimica Acta Part A: Mol. Biomol. Spectrosc. 2023, 298, 122766. DOI:10.1016/j.saa.2023.122766