Special Issues-10-01-2013

Recent advances in sampling techniques in the pharmaceutical industry sparked significant interest in applying improvements to extraction methods for greater analyte detection and quantitation.

Redox reactions are integral parts of many cellular processes. Thus, they are extensively studied in vitro and in vivo. Electrochemistry (EC) represents a pure instrumental approach to characterize direct and indirect effects of redox reactions on bioorganic molecules.

5-Fluorouracil (5-FU) is a low-molecular-weight anticancer drug in clinical use for several solid tumors in humans. Currently, the most widely used methodology for 5-FU quantitation is liquid chromatography–tandem mass spectrometry (LC–MS-MS) with either liquid–liquid extraction (LLE), protein precipitation, or a combination of both as sample cleanup procedures.

Gas chromatography with electron ionization and mass spectrometry (GC–EI-MS) detection is a workhorse among analytical techniques in metabolomics. A major challenge in the utilization of GC–EI-MS in metabolomics is the identification of unknowns.

Click the title above to open the October 2013 issue of Current Trends in Mass Spectrometry, Volume 11, Number 4, in an interactive PDF format.