November 2006. Raman spectroscopy is a promising new tool for noninvasive, real-time diagnosis of tissue abnormalities. Here, we show evidence of its application for cancer diagnosis in four distinct tissue types: skin, breast, gastrointestinal tract, and cervix. Multivariate statistical analysis and discrimination algorithms allow for automated classification of the spectra into clinically relevant pathological categories using histology as a gold standard. Although limitations exist, the technique shows every indication of being an exciting prospect in the management of cancer in a clinical setting.