Analytical Tools for the Characterization of Biopharmaceuticals: Chromatographic Methods


Spectroscopy E-Books

Spectroscopy E-BooksSpectroscopy E-Books-11-01-2014
Volume e2
Issue 3

Historically, ion-exchange chromatography and size-exclusion chromatography (SEC) have been the reference techniques for the physicochemical characterization of biopharmaceutical compounds. However, reversed-phase liquid chromatography is naturally more compatible with mass spectrometry detection. This article discusses new trends in the use of ion-exchange chromatography and SEC, as well as the power of using ultrahigh-pressure liquid chromatography (UHPLC) and advanced reversed-phase LC columns, including sub-2-µm wide-pore porous particles or core–shell particles, for biopharmaceutical characterization.   Read more


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