Garth Simpson of Purdue University previews an upcoming chirality session at the Eastern Analytical Symposium and explains why the meeting’s blend of academic and industry researchers produces the most impactful science.
|Articles|November 1, 2014
- Spectroscopy E-Books-11-01-2014
- Volume e2
- Issue 3
Analytical Tools for the Characterization of Biopharmaceuticals: Chromatographic Methods
Author(s)Davy Guillarme
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Historically, ion-exchange chromatography and size-exclusion chromatography (SEC) have been the reference techniques for the physicochemical characterization of biopharmaceutical compounds. However, reversed-phase liquid chromatography is naturally more compatible with mass spectrometry detection. This article discusses new trends in the use of ion-exchange chromatography and SEC, as well as the power of using ultrahigh-pressure liquid chromatography (UHPLC) and advanced reversed-phase LC columns, including sub-2-µm wide-pore porous particles or core–shell particles, for biopharmaceutical characterization. Read more
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