Davy Guillarme

	Historically, ion-exchange chromatography and size-exclusion chromatography (SEC) have been the reference techniques for the physicochemical characterization of biopharmaceutical compounds. However, reversed-phase liquid chromatography is naturally more compatible with mass spectrometry detection. This article discusses new trends in the use of ion-exchange chromatography and SEC, as well as the power of using ultrahigh-pressure liquid chromatography (UHPLC) and advanced reversed-phase LC columns, including sub-2-µm wide-pore porous particles or core–shell particles, for biopharmaceutical characterization.	 	Read more 

Davy Guillarme

Articles

Analytical Tools for the Characterization of Biopharmaceuticals: Chromatographic Methods