
|Articles|March 1, 2017
- Spectroscopy eBooks-03-01-2017
- Volume e5
- Issue 1
Discrimination of Bacterial Growth Media Using Portable Raman Spectroscopy with Background Fluorescence Subtraction
Author(s)Mathew G. Lyman, Jessica A. Randall
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We investigated the feasibility of identifying different types of bacterial growth medium using a handheld Raman instrument with a proprietary baseline correction algorithm. Our data suggest that this instrument is capable of differentiating multiple types of growth medium and readily detects the change in the Raman signal when bacteria are present in the growth medium. These findings support the notion that a handheld Raman spectrometer may be used to distinguish highly-fluorescent biological samples without the use of surface-enhanced Raman scattering.
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The Versatility of Portable Raman in Process DevelopmentAdvertisement
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Raman spectroscopy is shedding its bulky, benchtop reputation as chip-scale spectrometers, tip-enhanced probes, and AI-native detectors push the technique into pockets, production lines, and single molecules. The result is an instrument category being rebuilt from the optics up, faster, smaller, deeper, and smarter than the Raman systems of even five years ago.
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