Spectroscopy SupplementsAdvances in UV-vis-NIR Spectroscopy

Volume 36

Issue S12

Pages: 21–29

**We proposed a new retrieving model (named CAB1) for estimating the chlorophyll content at leaf level using the Prospect radiative transfer (RT) model. CAB1 is built based on multiband information derived from the radiative transfer model. Specifically, a continuous wavelet analysis algorithm was used to find the wavelet coefficients for those spectral characteristics that can highlight the chlorophyll content. Then, the simulated data sets generated by the Prospect model were used to evaluate the sensitivity of CAB1 in chlorophyll and its stability to other biochemical components (leaf structural parameters, water, carotenoids, brown pigment, and dry matter). Finally, CAB1 was verified using the Lopex93 and Angers experimental spectral data sets. Quantitative and qualitative results revealed that the retrieving model was not only more accurate than the traditional spectral index (the highest R^{2} of the inversion value and the measured value is 0.9), but also more stable.**

Chlorophyll is the dominant factor affecting vegetation photosynthesis, and it is closely related to other biochemical parameters such as protein, nitrogen, lignin, and water (1). The chlorophyll content in a crop can be used to indicate the photosynthetic capacity, growth cycles, and degrees of stress (such as disease, insect pests, and heavy metal stress) (2,3). Traditional biochemical methods for measuring the chlorophyll content (such as spectrophotometry, in which samples must be pretreated in the laboratory) are destructive to the vegetation as well as time-consuming and laborious; thus, spectral reflectance is an attractive alternative (4,5).

During the past few decades, many studies have proposed the spectral indices required to estimate chlorophyll contents. By using one of the existing spectral indices or the sensitive bands of chlorophyll, a single or multivariate analysis model can be established. Several studies have successfully estimated the chlorophyll content in vegetation using visible ratios (6), vis-NIR ratios (7–9), red-edge reflectance ratio indices (10,11), and spectral and derivative red-edge indices (12). Yan used the Prospect model to test the applicability of four types of spectral indices, such as visible ratios (eight kinds), vis-NIR ratios (eight kinds), red-edge reflectance ratio indices (six kinds), and derivative red-edge indices (six kinds), to test chlorophyll content extraction. She found that some spectral indices, such as normalized difference vegetation index (NDVI) and the modified chlorophyll absorption in reflectance index (MCARI), are not suitable for retrieving chlorophyll based on the reflectance spectra. Rather, it was necessary to carefully select a spectral index for a specific application (13,14). Using a spectral index to evaluate the chlorophyll content of vegetation is simple and flexible, but there are obvious limitations. One limitation is that the spectral index method does not consider the radiative transfer (RT) mechanism of light in the leaf, which leads to a lack of definite physical meaning (15). The other limitation is that spectral index models are generally adapted to specific databases and are difficult to generalize to other databases.

Therefore, researchers began to consider optical models with geometric meanings, including the radiative transfer performance of light in vegetation, and the effects of vegetation biochemistry on light. The process of light reflection and absorption in vegetation and the interaction process were mathematically described to retrieve the biochemical components of the vegetation. Optical models, such as the Prospect (16), Liberty (17), and Leafmod (18) models, are not limited to the time, location, and other factors, and they are robust to noise. Yan noted that the reflection and absorption spectra simulated using the Prospect model are nearly the same as those of the measured spectra. Jiang used the Prospect model to simulate spectral data of different spectral scales (different bandwidths (5–65 nm)) and analyzed the validity and sensitivity when applied to the estimation of the chlorophyll content (19). Wang used the Prospect model to simulate the spectra of leaves with different biochemical characteristics, and analyzed the influence of different mathematical combinations of the NDVI spectral index on the elimination of interference factors. The spectral index of the dual NDVI ratio vegetation index was established to estimate the carotenoid content in leaves (20). Zhang used the Prospect model to construct a lookup table from which the chlorophyll content of eucalyptus leaves could be retrieved. The spectral data determined using a statistical method, an estimation model linking the chlorophyll, and carotenoid content with spectral characteristic parameters of eucalyptus leaves, were established (21).

The aim of this study was to construct a new spectral index for estimating chlorophyll content at the leaf level with physical implications, robustness, and universality. First, the relationship between the chlorophyll content and the spectral characteristics of vegetation based on the transmission mechanism of the Prospect optical model was analyzed to construct a new specific band. The “Experimental Materials and Methods” section introduces the experimental materials and methods, and describes the establishment of a new inversion model for chlorophyll content with continuous wavelet transform (CWT). The “Results” section reviews the experimental results, which describe the new inversion model validation of comparison with other vegetation indices. The “Discussion” section focuses on the advantages and disadvantages of new spectral index. Finally, the “Conclusion” section summarizes the findings of the study.

The Prospect model, among the most mature RT models, has few required parameters and easy inversion. It can simulate the optical properties of vegetation leaves from 400 nm to 2500 nm (22). The Prospect model is a simple “flat model.” The leaf is composed of *N*-plate layers and *N*-1 air layers. In Prospect-5 (23), the total spectral absorption coefficient (*k*) of each leaf plate layer is a function of leaf structural parameters (*N*), chlorophyll content (*C _{ab}*), water content (

where *K _{e}* is the refractive index of the basic whitening layer;

*K _{e}*,

According to different experimental purposes, the input parameters of the Prospect model are set, and the simulated data set with different biochemical components can be simulated. Four simulation data used in this paper, named Set_*C _{ab}*, Set_

The sensitivity index, SI, is used for accuracy evaluation (equation 2) (26).

where *VI* is the calculated values of the spectral indices and |*VI*| is the absolute value. The smaller the *SI*, the smaller the influence of the component on the spectral index.

The Lopex93 database was established by the Joint Research Center (JRC) in 1993 (27). Approximately 70 leaf samples representations of more than 50 species were obtained. The database contains 62 samples of spectral data and chlorophyll content data, including 13 monocotyledons and 49 dicotyledons. Spectra were collected over the 400–2500 nm region with a sampling interval of 1 nm. The database not only takes into account the differences of different species, but also the changes of biochemical composition in time. It fully comprises the diversity of biochemical composition content and inner types of leaves. The database cannot only provide a more effective test for spectral index, but also serve as an ideal data source for analyzing the correlation between chlorophyll content and spectral reflectance of mixed species. The Lopex93 data set can be accessed free online (http://opticleaf.ipgp.fr/index.php?page=database).

The Angers database was established at the National Institute for Agricultural Research (INRA) in Angers, France, in June 2003 (23). Approximately 276 leaf samples representations of more than 39 species were obtained. The directional-hemisphere reflectance spectra were measured using an ASD FieldSpec spectrometer with a spectral range of 400–2500 nm and a data interval of 1.4 nm. The biochemical parameters include leaf chlorophyll content, carotenoids, specific leaf weight (SLW), and water content. The Angers data set can also be accessed free online (http://opticleaf.ipgp.fr/index.php?page=database).

Wavelet transform is an effective mathematical tool used to decompose the original spectral signal into multiple scales (28,29). The continuous wavelet transform (CWT) tool can make the wavelet shift smoothly to different positions; thus, each scale component can be compared directly to the input spectral reflectance, and more useful spectral information can be captured (30). In this study, the CWT wavelet analysis method was used in the spectral analysis method. The CWT is defined as follows in equation 3 (31),

where *W*_{f} is the signal to be analyzed; Ψ is a chose wavelet basis function, such as biorthogonal (bior), daubechies (db), coiflets (coif), or symlets (sym) (32); Ψa,b (λ) is the prototype to generate child wavelets by adjusting the scale parameter *a* (the corresponding decomposition frequency or band range) and b the translation factor (corresponding to the band position); λ is the wavelength; and dλ is the spectral resolution.

In this study, the spectral reflectance signal was processed by continuous wavelet transform, where f(λ) is the spectral reflectance value or absorption coefficient spectrum of the leaf. Commonly used continuous wavelet decompositions are selected as Ψ; a is the spectrum scale of 100 nm, 150 nm, and 200 nm; *b* is the band position which should be selected by analysis; λ is the wavelength that can be cut down by analysis; and dλ = 1 nm. After the CWT wavelet analysis, the coefficients are obtained, which can be used for singularity detection or periodic analysis. When the signal is projected into the wavelet transform domain, it is advantageous to extract some useful features.

To construct the new spectral index for the chlorophyll content of leaves, the relationship between the reflectance and biochemical components of the vegetation is built first. As is known, the summation of the spectral reflectance of the leaf (*R*), transmittance (*T*), and absorption (*A*), were constantly one. The shape of the leaf reflectance and transmittance were found to be similar in the visible and near- infrared (NIR) radiation, and could be expressed approximately as follows (33):

where α is a constant.

The spectrum absorption rate, *A*, could be expressed as:

By combining equation 1 with equation 5, the formula of the leaf spectrum reflectance rate (*R*) could be obtained.

Different biochemical components have a different influence on the reflectance in the band 400–800 nm (the sensitive spectrum range of the chlorophyll). In the Prospect model, only one biochemical component was set with different ranges and the other input parameters were fixed to obtain many reflectance spectra, which were used to test the influence of the biochemical component on the reflectance. For example, to test the *C _{ab}* in the band 400–800 nm influence on the reflectance in the Prospect model,

where *b* is a constant value, meaning *C _{m}*,

In mathematics, the result of CWT computation for constant value is 0. The calculated value of constant coefficients with CWT computation remains unchanged. Therefore, both sides of equation 7 are carried out with the CWT computation. At the same time, we can obtain equation 8

where the wavelet coefficients W_{R}(a,b), W_{ke}(a,b) and W_{kab}(a,b) are determined by scaling factor *a*, the specific band position *b*, and wavelet base function Ψ.

Finally, two factors were considered to determine the scale basis function and band position of the continuous wavelet decomposition in building the new spectral index. One factor was in the range of 550–700 nm, the CWT curve of the *k _{ab}*(a,b) should have a wave valley or wave peak at bands w1 and w2, where the chlorophyll absorption was sensitive. The other factor was that the CWT curve of

At the wave-peak w1 and wave-valley w2, two formulas can be obtained using Formula 8.

where w1 equals 699 nm, w2 equals 613 nm, *a* equals 150 nm, and the wavelet function is “rbio1.1”. According to Figure 2, we can obtain the wavelet coefficients *W _{kab}* and

A new leaf chlorophyll content model, termed CAB1 with the wavelet function “rbio1.1,” was obtained as equation 12:

CAB1 is only affected by the parameter *N*. Then, for the same crop type during the same growth period, *N* was a fixed constant and CAB1 was only affected by the leaf spectrum reflectance rate.

To test the established inversion model CAB1, eight commonly used spectral indices (Table II) sensitive to leaf chlorophyll contents were used for comparison, including the spectral index of visible light NPCI, TCARI, and MCARI; spectral indices of visible and infrared light MTCI, TVI, and TCARI/OSAVI; and red-edge spectral index GM and VOG2.

Two aspects should be considered in the spectral index evaluation: The spectral index is sensitive to chlorophyll content, and it can resist the influence of other factors. First, the Prospect optical model was used to simulate the spectral curves with different chlorophyll content, and the advantages and disadvantages of each spectral index were analyzed. According to existing spectral indices (Table II), the band range was selected from 400 to 800 nm. According to the analysis in Figure 1, the influencing factors only included *N*, *C _{m}*, and

The data of Set_*C _{ar}* is used for spectral indices sensitivity for leaf chlorophyll content

The test result of Set_*N* is shown in Table III (the second line). The influence of *N* on the partial spectral index is large, such as NPCI, TCARI, MTCI, and TCARI/OSAVI. Under the same chlorophyll content, the MTCI value changed approximately 19 times when the N changed from 1 to 3, followed by NPCI, TCARI, and TCARI/OSAVI. The SI of VOG2, GM, and CAB1 are smaller, particularly the SI value of CAB1, which is only 34.87%.

The test result of Set_*C _{m}* is shown in Table III (the third line). The influence of

The test result of Set_*C _{ar}* is shown in Table III (the fourth line). The influence of

Using the 190 Lopex93 reflectivity spectral data, the quadratic model of CAB1, TCARI, MTCI, and VOG2 were built. The 80 validation spectral data were used to verify the inversion chlorophyll content accuracy. For Angers data, 69 reflectivity spectral data were used to build the quadratic model of CAB1, TCARI, MTCI, and VOG2, and 60 validation spectral data were used as validation data. Because the structural parameter of the leaf *N* cannot be determined in the Lopex93 and Angers data, *N* is assumed to be the standard value, which is 2. The *R*^{2} and RMSE are used to evaluate their accuracy (Table IV). Validation results of chlorophyll estimations are done by the quadratic model of CAB1, TCARI, MTCI, and VOG2, which is shown in Figure 4.

As shown in Table IV and Figure 4, the quadratic models of CAB1, TCARI, MTCI, and VOG2 with Lopex93 data have low RMSE (RMSE < 3.20). The *R*^{2} of MTCI is greater than 0.72, which is better than TCARI and VOG2. The *R*^{2} of CAB1 is 0.9108, and the RMSE values are smaller (RMSE = 2.0294). Compared with TCARI, MTCI, and VOG2, CAB1 is more accurate and robust.

The results of CAB1, TCARI, MTCI, and VOG2 estimating chlorophyll with Angers data are shown in Table IV and Figure 4. The quadratic models of CAB1, TCARI, MTCI, and VOG2 with Lopex93 data have high *R*^{2} (*R*^{2} > 0.84), which indicates that they have high accuracy for chlorophyll estimation. The *R*^{2} of MTCI is greater than 0.89, which is better than TCARI and VOG2. The *R*^{2} of CAB1 is 0.9116, and the RMSE values are smaller (RMSE = 3.6925). Compared with TCARI, MTCI, and VOG2, CAB1 is more accurate and robust.

From the analysis of two sets of data, it was found that the *R*^{2} of CAB1 was the highest, and the RMSE was the smallest among the four spectral indexes, indicating that CAB1 performs the best.

As shown in equation 8 using the spectral analysis method, the spectral reflectance is limited to 700–800 nm, and the inversion of chlorophyll content has only two important factors: *N* and *C _{ab}*. By using continuous wavelet decomposition, the spectral energy can be decomposed to find the band position with the largest

The Prospect model starts with spectral and vegetation components and has a strong theoretical basis. Therefore, the spectral index is constructed step-by-step based on the Prospect formula, which has sufficient theoretical basis. However, the results are still affected by *N* (equation 12). Although the experimental results show that the effect of *N* on CAB1 is relatively small, the accuracy of vegetation index would be further improved if the specific value of *N* can be obtained from the measured data. In addition, the model used in this experiment was Prospect-5, which does not separate chlorophyll from anthocyanin, resulting in a greater impact of *C _{ar}*, shown in Table III (the fourth line). With the refinement of the Prospect model, the accuracy of building a new vegetation index is expected to be further improved.

From the Prospect simulation data, CAB1 is in the upper and middle reaches, shown in Table III. Through the aforementioned analysis, the spectral index on the sensitivity of chlorophyll content and ability to resist the influences of other components such as the structure of mesophyll, dry matter, and carotenoids, were summarized to estimate the effectiveness of assessing chlorophyll content. The comprehensive evaluation index (*Ava*) is defined as follows:

where *R*^{2} is the spectral sensitivity of the biochemical components, as represented by the index trend line fitting degree value *R*^{2} (Table III); 1 − SI% is the ability of spectral indices to resist other biochemical components (SI% are selected in Table III); *P*, *P _{i}* ...

Table III shows that NPCI, TCARI/ OSAVI, and MCARI have the low *Ava* value and are not suitable for evaluating chlorophyll content in a vegetation leaf. MTCI and TVI comprehensive evaluations are medium, and they have a certain guiding role for the particular vegetation or a certain period of growth of vegetation but are not used for all vegetation. The *Ava* values of CAB1, GM, and VOG2 are higher (greater than 0.8), and the models are sensitive to chlorophyll content and are relatively stable. The CAB1 integrated capacity evaluations were equivalent to model sensitivity and stability optimum. CAB1 is based on the Prospect model, so using Prospect simulation data can explain the status quo of the vegetation index. Although CAB1 does perform relatively well based on the Lopex93 and Angers data validation, more measured data are needed for further validation.

This study generated a new spectral index, named CAB1, to estimate the chlorophyll content of a vegetation leaf. Based on the Prospect optical model, the continuous wavelet analysis method was used to establish the CAB1 model, in which the “rbio1.1” wavelet basis function with a scale of 150 nm, as well as reflectance spectra for 613 and 619 nm, were selected. The CAB1 model is derived from the Prospect model, so the CAB1 model has a strict physical meaning. The practical adaptability of some spectral indices, which are based on statistics, was the biggest disadvantage. CAB1 is not based on statistics, but instead on the theory of real light propagation, which is more adaptable.

A good chlorophyll vegetation index should be sensitive to chlorophyll while simultaneously not being sensitive to other factors. To verify the adaptability of CAB1, a comparison was made with eight commonly used spectral indices. According to the Prospect model, the data describing the relationship between the leaf spectrum and the content of each component were simulated. A comparison of the chlorophyll sensitivity and resistance to the mesophyll structure, dry matter, and carotenoid content of the CAB1 model and the common chlorophyll spectral indices was examined. MCARI was found to perform the worst (*Ava* = -3.7600). MCARI is very sensitive to the chlorophyll content but also to other components such that it is only suitable for retrieving the chlorophyll content of the same vegetation leaf within a single period. The VOG2, GM, and CAB1 models performed the best (*Ava* > 0.8) because they can retrieve the chlorophyll content of any vegetation leaf with high accuracy. To verify this conclusion, the actual data of Lopex93 and Angers were selected. CAB1 (proposed method), TCARI (spectral index of visible light), MTCI (spectral indices of visible and infrared light), and GM (red edge spectral index) were selected to retrieve the chlorophyll content of vegetation leaves using the Lopex93 and Angers data. Based on the actual data, MTCI, TCARI, and VOG2 were found to have moderate inversion accuracy (the maximal *R*^{2} is 0.8), which may be because of noise in the actual data. However, CAB1 offers a high level of accuracy (*R*^{2} > 0.9), which indicates the CAB1 model is not only more accurate than the other vegetation indexes, but is also very stable. Overall, CAB1 performed well in the leaf layer compared to the commonly used spectral indices NPCI, TCARI, and MCAI in visible regions, and CAB1 is comparable to the red edge spectral index GM and VOG2.

This research was based on the Prospect model simulation data. Because of the influence of the accuracy of the model, and given that the experimental data may be slightly different, the universality of the research results must be further verified. However, the results of this study provide some pointers for combining the physical model and the spectral index method.

This work was supported by the Joint Innovative Center for Safe and Effective Mining Technology and Equipment of Coal Resources, Shandong Province, and Scientific Research Foundation of Shandong University of Science and Technology under Grant [2014TDJH101]; Scientific and Technological Planning Projects of Colleges and Universities in Shandong Province under Grant [J18KA214]; and Funded by Beijing Key Laboratory of Urban Spatial Information Engineering [2019209].

We thank the SDUST Laboratory for the use of their equipment. The authors also thank the editor and anonymous referees for their insightful comments, which improved this paper.

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**Feifei Xie** and **Lin Sun** are with the State Key Laboratory of Mining Disaster Prevention and Control at Shandong University of Science and Technology in Qingdao, China. **Xie** and **Fengzhu Liu** are with the Beijing Key Laboratory of Urban Spacial Information Engineering, in Beijing, China. **Jie Wang** is with Inner Mongolia University in Hohhot, China. **Liu** is also with the Beijing Institute of Surveying and Mapping, Beijing, China. Direct correspondence to: xff@sdust.edu.cn ●

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