Scientists at Kinexus Bioinformatics Corporation (Vancouver, British Columbia, Canada), in collaboration with researchers at the Centre for High Throughput Biology at the University of British Columbia (Vancouver, British Columbia, Canada), investigated the host signaling pathways that are affected by Salmonella enterica, a food-borne bacteria that is a leading cause of food poisoning in North America and around the world.
Scientists at Kinexus Bioinformatics Corporation (Vancouver, British Columbia, Canada), in collaboration with researchers at the Centre for High Throughput Biology at the University of British Columbia (Vancouver, British Columbia, Canada), investigated the host signaling pathways that are affected by Salmonella enterica, a food-borne bacteria that is a leading cause of food poisoning in North America and around the world. The results of the study, using mass spectrometric analyses, revealed that more than 24 percent of 9500 phosphorylation sites tracked in human cells were significantly altered with 20 minutes of Salmonella infection.
Application of Kinexus’s Kinase Predictor algorithm for 493 human protein kinases against each of the top Salmonella-affected phosphosites permitted identification of specific protein kinases that are affected by Salmonella, including the proto-oncogene-encoded protein kinases Pim1. Specific inhibition of Pim1 in follow-up studies was found to mitigate some of the pathogen effects of this bacteria, and implicated this kinase as a possible target for therapeutic drug intervention.
More than 100,000 human phosphosites in more than 14,000 of the 23,000 proteins encoded by the human genome have now been experimentally confirmed. This study has led to the identification of over 6000 previously unknown phosphosites, and these have been posted for open access on the Kinexus PhosphoNET website www.chromatographyonline.com
Getting accurate IR spectra on monolayer of molecules
April 18th 2024Creating uniform and repeatable monolayers is incredibly important for both scientific pursuits as well as the manufacturing of products in semiconductor, biotechnology, and. other industries. However, measuring monolayers and functionalized surfaces directly is. difficult, and many rely on a variety of characterization techniques that when used together can provide some degree of confidence. By combining non-contact atomic force microscopy (AFM) and IR spectroscopy, IR PiFM provides sensitive and accurate analysis of sub-monolayer of molecules without the concern of tip-sample cross contamination. Dr. Sung Park, Molecular Vista, joined Spectroscopy to provide insights on how IR PiFM can acquire IR signature of monolayer films due to its unique implementation.
Researchers Develop Adaptive Gap-Tunable SERS Device
April 24th 2024In a new study, researchers from Ulsan National Institute of Science and Technology and Pohang University of Science and Technology presented a new surface-enhanced Raman spectroscopy (SERS) device, improving gap plasmon resonance.