Wavelength Tech Forum: Fluorescence
This month's Technology Forum looks at the topic of fluorescence and the trends and issues surrounding it. Joining us for this discussion is Paul Orange, Strategic Development Leader, Cellular Imaging and Analysis, PerkinElmer Life and Analytical Sciences.
This month's Technology Forum looks at the topic of fluorescence and the trends and issues surrounding it. Joining us for this discussion is Paul Orange, Strategic Development Leader, Cellular Imaging and Analysis, PerkinElmer Life and Analytical Sciences.
What role will imaging and microscopy play in the future of the fluorescence industry?
(Orange) Huge. Nano-tech and reducing sample sizes will mean that high sensitivity detection devices are required to evaluate samples.
How do you think fluorescence will advance the field of clinical diagnostics?
(Orange) If used with living materials, then there is a massive impact, as many sub-cellular processes will be only visible when used with fluorescent dyes. Fluorescent dyes are already used in a clinical setting for genetic analysis, such as FISH. The likely increase of genetic analysis of clinical samples will inevitably lead to an increased demand for fluorescent technologies.
How has fluorescence had an impact on biosample analysis?
(Orange) Yes, a massive impact. Fluorescence transgenic proteins have really allowed researchers to get detailed information on the activity of subcelluar components, down to the level of individual protein species. The spectral separation of the dyes used allows multiplexing (in fluorescence microscopy, typically up to 4-plex), which when used in techniques such as FRET, allow the interactions between different proteins to be studied. Clearly the sensitivity of the dyes also has a huge impact, as they allow us to see small quantities of the analyte.
What new developments in fluorescence have you most intrigued?
(Orange) Photoswitchable and photoactivateable proteins are very interesting in biological settings as they give the ability to get more information out of a sample, and will allow a single molecule or to be studied, against a background of the entire cell. I'm also interested in compounds that enable FLIM analysis; these really take interaction studies to the next level.
What do you think?
Click here to participate in our "Question of the Month" survey on fluorescence.
Getting accurate IR spectra on monolayer of molecules
April 18th 2024Creating uniform and repeatable monolayers is incredibly important for both scientific pursuits as well as the manufacturing of products in semiconductor, biotechnology, and. other industries. However, measuring monolayers and functionalized surfaces directly is. difficult, and many rely on a variety of characterization techniques that when used together can provide some degree of confidence. By combining non-contact atomic force microscopy (AFM) and IR spectroscopy, IR PiFM provides sensitive and accurate analysis of sub-monolayer of molecules without the concern of tip-sample cross contamination. Dr. Sung Park, Molecular Vista, joined Spectroscopy to provide insights on how IR PiFM can acquire IR signature of monolayer films due to its unique implementation.
Deep Level Transient Spectroscopy Reveals Influence of Defects on 2D Semiconductor Devices
April 25th 2024A recent study used deep level transient spectroscopy to investigate the electrical response of defect filling and emission in monolayer metal-organic chemical vapor deposition (MOCVD)-grown materials deposited on complementary metal-oxide-semiconductor (CMOS)-compatible substrates.
Single Cell and Microplastic Analysis by ICP-MS with Automated Micro-Flow Sample Introduction
April 25th 2024Single cell ICP-MS (scICP-MS) is increasingly seen as a powerful and fast tool for the measurement of elements in individual cells, mainly due to the high sensitivity and selectivity of ICP-MS. Analysis is performed in the same way as single nanoparticle (spICP-MS) analysis, which has become a well-established technique for the analysis of nanoparticles and particles.
Hot News on Agilent LDIR, New Developments, and Future Perspective
April 25th 2024Watch this video featuring Darren Robey and Dr. Wesam Alwan from Agilent Technologies to gain insights into the future trends shaping microplastics research and the challenges of their characterization. Discover the essential components necessary for accurate microplastics analysis and learn how the Agilent 8700 LDIR system addresses these challenges. Offering rapid and precise analysis capabilities, along with easy sample preparation methods that minimize contamination, the Agilent 8700 LDIR system is at the forefront of advancing microplastics research.
