Author | Jennifer Krone

Jennifer Krone is with Hitachi High-Technologies America, San Jose, California.

Articles

Dynamic Range Improvements on the Hitachi NanoFrontier Linear Ion Trap-TOF LC-MS Platform

March 01, 2007

Application Notebook

Article

Hitachi High Technologies America, Inc.

Identification of a Low-Abundant Biomarker in Human Serum Using nanoLC–LIT-TOF MS and Information-Based Acquisition Techniques

November 01, 2006

Special Issues

Article

Serum protein profiling using mass spectrometry (MS) is one of the most promising approaches for biomarker identification. The authors adopted a nano liquid chromatography (nLC)–linear ion trap time-of-flight (LIT-TOF) MS system and newly developed software known as information-based acquisition (IBA) to identify biomarkers in human serum. IBA is a data processing protocol for repetitive MS analyses. Peptides selected for the first-pass MS-MS analysis are automatically excluded from the MS spectrum such that subsequent MS-MS analyses are performed on different peptides to minimize overlapping analyses, resulting in the identification of relatively low-abundant peptides.