Special Issues-03-01-2011
Special Issues
25-Hydroxyvitamin D2/D3 Analysis in Human Plasma Using LC–MS
March 01, 2011
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Increases in vitamin D testing frequency have required more rapid and cost-effective solutions for determining vitamin D levels in plasma. This LC–MS method was adapted for use with core-shell columns to achieve run times of less than 4 min.
Comparison of Extracts from Dry and Alcohol-Steamed Root of Polygonatum kingianum (Huang Jing) by Sub-2-µm–LC–TOF-MS
March 01, 2011
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Most plants used in traditional Chinese medicine must be processed before their medicinal usage; hence the effective ingredients may differ from those in the freshly harvested plant extracts. In this work, we present a fast and generic approach using sub-2-?m liquid chromatography–time-of-flight–mass spectrometry (sub-2-?m-LC–TOF-MS) coupled with multivariate statistical data analysis to systematically profile ingredient changes between fresh and processed samples of huang jing.
Mass Spectrometry Advances Fossilomics
March 01, 2011
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Fossilomics uses MS to extract amino acid sequence information from subpicomole quantities of protein and peptide fragments that remain in certain fossil samples. The sequences are compared to databases and validated with search statistics and high-confidence sequences. The validated sequences can then be used to place the fossils on the evolutionary tree.
Time-Resolved SRM Analysis and Highly Multiplexed LC–MS-MS for Quantifying Tryptically Digested Proteins
March 01, 2011
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The inclusion of time-resolved selected reaction monitoring (SRM) functionalities into mass spectrometer control software allows large numbers of peptides to be quantified using short LC–MS-MS methods.
Responding to Data Analysis and Evaluation Challenges in Mass Spectrometry–Based Methods for High-Throughput Proteomics
March 01, 2011
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This new data-management method is specifically designed to handle the large amounts of data produced in proteomics studies. Experimental methods and data are presented from a recent study conducting proteomics profiling of Schizosaccharomyces pombe to demonstrate the effectiveness of the data management solution. The results demonstrate enhanced data search speeds as well as effective data analysis.
Metabolomics Workflows: Combining Untargeted Discovery-Based and Targeted Confirmation Approaches for Mining Metabolomics Data
March 01, 2011
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The metabolomics workflow described here combines untargeted (discovery) quadrupole time-of-flight (Q-TOF) liquid chromatography–mass spectrometry (LC–MS), targeted (confirmation) triple-quadrupole LC–MS-MS, and sophisticated data mining as an effective means to elucidate metabolite changes.
Vol 9 No 1 Current Trends in Mass Spectrometry March 2011 Issue PDF
March 01, 2011
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Click the title above to open the March 2011 issue of Current Trends in Mass Spectrometry, Volume 9, Number 1, in an interactive PDF format.