Martin Vollmer


On-line and Off-line 2-D LC–ESI MS-MS Methods in Proteomic Analysis

May 02, 2005

Off-line 2-D LC–MS-MS represents a powerful alternative to on-line methodologies for protein identification from complex proteomes, improving the chromatographic resolution of digest peptide mixtures, even for low-abundance proteins. Here, the authors provide a detailed comparison of the two techniques.