Author | Parastoo Azadi

Articles

Mass Spectrometry Techniques to Unravel the Heterogeneity of Glycoproteins

October 01, 2017

Special Issues

Article

Since glycans are responsible for bioactivity, solubility, immunogenicity, and clearance rate from circulation, it is vital to have a detailed map of glycans in therapeutic glycoproteins. Detailed glycoprotein structural analysis must be able to identify the peptide sequence where the glycans are attached as well as the structure of the glycan portion, including oligosaccharide sequence and glycosyl linkages. This article details methods for mass spectrometry experiments on both released glycans (“glycomics”), as well as on intact glycopeptides (“glycoproteomics”) using electron transfer dissociation, high-energy collision dissociation, and collisioninduced dissociation fragmentation pathways, which are needed to fully elucidate the structure of glycoproteins.

Analyzing Carbohydrates on Therapeutic Glycoproteins

November 01, 2014

Spectroscopy E-Books

Article