SPEC PerkinElmer 11.10

Fluorescence

Latest News


Micrography, microphotography of vesicles, particles or green fluorescent cells for scientific research, taken under a high-tech microscope. Fluorescence of curcumin and other fluorophores of interest | Image Credit: © DavidBautista - stock.adobe.com

A theoretical study explores the excited state behavior of triazole pyrimidine group fluorophores, revealing insights into their fluorescence mechanisms and the absence of excited state intramolecular proton transfer (ESIPT). The findings contribute to the understanding and potential applications of these fluorophores in various scientific disciplines.

Förster resonance energy transfer (FRET) is a versatile part of the toolbox of fluorescence methods. This through-space, photon-less energy transfer process between a donor fluorophore and an acceptor chromophore is perhaps most famous for its utility as a “molecular ruler” that can resolve nanometer-scale distances. FRET is also a popular and advantageous basis for biomolecular assays and sensors.

Tunable narrowband light sources are essential for measuring fluorescence, reaction energetics, and other challenging measurements in chemical, biological, and physical systems. In particular, the optical parametric oscillator (OPO) is an indispensable tool.