Fluorescence

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Förster resonance energy transfer (FRET) is a versatile part of the toolbox of fluorescence methods. This through-space, photon-less energy transfer process between a donor fluorophore and an acceptor chromophore is perhaps most famous for its utility as a “molecular ruler” that can resolve nanometer-scale distances. FRET is also a popular and advantageous basis for biomolecular assays and sensors.

Tunable narrowband light sources are essential for measuring fluorescence, reaction energetics, and other challenging measurements in chemical, biological, and physical systems. In particular, the optical parametric oscillator (OPO) is an indispensable tool.