UV-vis Spectroscopy

August 19th 2025

This tutorial investigates the persistent issue of sample heterogeneity—chemical and physical—during spectroscopic analysis. Focus will be placed on understanding how spatial variation, surface texture, and particle interactions influence spectral features. Imaging spectroscopy, localized sampling strategies, and adaptive averaging algorithms will be reviewed as tools to manage this problem, as one of the remaining unsolved problems in spectroscopy.

August 11th 2025

Researchers at Santiago de Compostela University (Santiago, Spain) find ultraviolet–visible (UV–vis) spectroscopy can detect and quantify post-COVID condition with high accuracy, paving the way for real-time clinical use.

August 11th 2025

A new study reveals that anthocyanin-rich fruit extracts degrade rapidly under sunlight but remain most stable in cold, dark storage.

August 11th 2025

Inter-instrument variability is a major obstacle in multivariate spectroscopic analysis, affecting the reliability and portability of calibration models. This tutorial addresses the theoretical and practical challenges of model transfer across instruments. It covers spectral variability sources—such as wavelength shifts, resolution differences, and line shape variations—and presents key standardization techniques including direct standardization (DS), piecewise direct standardization (PDS), and external parameter orthogonalization (EPO). We discuss the underlying mathematics of these approaches using matrix notation and highlight limitations that must be considered for reliable universal calibration.