A recent study used combined chemical analysis approach to investigate rat brains harvested one week after induction of photothrombotic stroke.
A recent study used combined chemical analysis approach to investigate rat brains harvested one week after induction of photothrombotic stroke (1). Researchers from Austria, the Netherlands, and Qatar used Fourier transform infrared (FT-IR) spectroscopy and laser ablation inductively coupled plasma–mass spectrometry (LA-ICP-MS) for the imaging of cerebral ischemia to obtain a deepened analysis compared to single techniques or side-by-side analysis.
The LA-ICP-MS data were normalized using an internal standard (a thin gold layer). Hyperspectral data cubes that were acquired were fused and subjected to multivariate analysis. Brain regions affected by stroke as well as unaffected gray and white matter were identified and classified using a model based on either partial least squares discriminant analysis (PLS-DA) or random decision forest (RDF) algorithms.
According to the researchers, the RDF classifier built on the combined data sets appeared to be more precise compared to classifiers built on the individual data sets. They concluded stated that multisensory hyperspectral imaging with subsequent RDF-based analysis can deepen the understanding of biochemical processes in ischemic brains and enables automated identification of different types of tissues.
Reference
Researchers Develop Adaptive Gap-Tunable SERS Device
April 24th 2024In a new study, researchers from Ulsan National Institute of Science and Technology and Pohang University of Science and Technology presented a new surface-enhanced Raman spectroscopy (SERS) device, improving gap plasmon resonance.
Getting accurate IR spectra on monolayer of molecules
April 18th 2024Creating uniform and repeatable monolayers is incredibly important for both scientific pursuits as well as the manufacturing of products in semiconductor, biotechnology, and. other industries. However, measuring monolayers and functionalized surfaces directly is. difficult, and many rely on a variety of characterization techniques that when used together can provide some degree of confidence. By combining non-contact atomic force microscopy (AFM) and IR spectroscopy, IR PiFM provides sensitive and accurate analysis of sub-monolayer of molecules without the concern of tip-sample cross contamination. Dr. Sung Park, Molecular Vista, joined Spectroscopy to provide insights on how IR PiFM can acquire IR signature of monolayer films due to its unique implementation.
The World of Microplastics Up to Date – an Overview
April 23rd 2024Watch this 20-minute educational video by Andreas Kerstan, Agilent Product Specialist in molecular spectroscopy, to gain a comprehensive update on the microplastics landscape and the environmental concerns related to them. Discover the current challenges in microplastics characterization and how Agilent innovative solutions and techniques, including FTIR, LDIR, GC/MS, and ICP-MS, are addressing these issues head-on.