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FT-IR Microscopy, Part 2: Mid-IR Sampling with DRIFTS, IRRAS, and ATRFourier transform infrared (FT-IR) microscopy using reflection methods (diffuse reflection, reflection/reflection-absorption, or attenuated total reflectance) typically requires less sample preparation than transmission. However, optimal results will depend upon the sample and, in particular, the sample surface.
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Time-resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisome of Thermophilic Cyanobacterium Thermosynechococcus vulcanus NIES 2134Time-resolved fluorescence spectroscopy reveals much about the structure-induced energy transfer mechanisms of phycobilisomes, the light-harvesting antenna in cyanobacteria.
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An Archaeometric Investigation into the Former Cataract House Hotel via Elemental AnalysisPortable X-ray fluorescence was used to analyze the archaeological remains of an Underground Railroad station to gain a clearer understanding of the construction phases it underwent during the 19th century.
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In situ Monitoring of Double Metal Cyanide (DMC) Catalyst Synthesis by Raman SpectroscopyDouble metal cyanide (DMC) can be monitored in real time using an in situ Raman spectroscopy method. In this study, real-time reaction progress information from in situ Raman results enabled researchers to accurately determine the reaction end point of DMC.
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Preparation and Spectral Properties of MgAl2O4: Tb3+ PhosphorA look at how the spectral properties single-phase green emission phosphor make it suitable for near-UV light-emitting diode (NUV-LED) applications.
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Aggregate and Fragment Analysis in Therapeutic Monoclonal Antibodies Using On-Line Size-Exclusion Chromatography with Native Mass SpectrometrySize-exclusion chromatography (SEC), with the use of ammonium acetate buffer, can be coupled on-line to electrospray ionization MS for the characterization of size variants of therapeutic monoclonal antibodies (mAbs). A quadrupole time-of-flight (QTOF) MS system was employed, and the MS method was optimized to achieve favorable sensitivity for high-mass detection, while maintaining the structural integrity of the aggregates (or high molecular weight species) and fragments (or low molecular weight species).
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Inversion of Low-Grade Copper Mining Areas Based on Spectral Information and Remote Sensing Data Using Vis-NIRDepletion of modern mineral resources due to continuous exploitation and utilization makes it economically necessary to quickly identify the locate sources of low-grade ore. Here, we propose a vis-NIR remote sensing method to determine copper content in mining areas as well as to measure the environmental impact of surface mining methods.
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Enhanced Raman and Mid-Infrared Spectroscopic Discrimination of Geographical Origin of Rice by Data Mining and Data FusionThe application of data mining combined with data fusion of Raman and mid- infrared spectra was studied to improve discrimination ability for modeling the geographical origins of rice.
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MilliporeSigma: Ultrapure Water for Regulated Metal Analysis of Food & Drinking WaterThis application note demonstrates the performance of a Milli-Q® ultrapure water system for the quality control of metals in food and water samples in regulated labs.
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A Different Kind of Art AnalysisRaman and XRF spectroscopy were used to examine paint in artwork, revealing the potential of both techniques to verify the authenticity of famous works of art.
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Extractable and Leachable Testing for Pharmaceutical Packaging, Finished Pharmaceutical Products, and Medical Devices: An Analytical PerspectiveExtractable and leachable (E&L) testing for finished pharmaceutical products, bioprocess manufacturing systems, and medical devices is currently a subject of intense interest. The majority of the challenges encountered in analytical workflows are related to the highly complex matrices and relatively low analyte evaluation thresholds seen in E&L studies. This paper provide options for executing E&L testing to supporting regulatory submissions. There are multiple compliant routes possible, and the presented workflows and analytical solutions are only one of multiple successful approaches.
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Specific Recognition Technology of Infrared Absorption Spectra Based on Continuous Wavelet DecompositionIR absorption spectroscopy technology can solve the problem of line aliasing in gas detection. Here, continuous wavelet transform was used in time-frequency analysis to improve spectral component identification and quantitative detection of gases.
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Terahertz Spectral Investigation of L-Cysteine Hydrochloride and its MonohydrateThis new terahertz method provides a theoretical reference for studying the relationship between biomolecules and water.
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EDX and LIBS Studies on Gallstones Using Partial Least Squares Discriminant Analysis (PLS-DA)Understanding gallstone formation requires examining their elemental composition. Here, EDS and LIBS were used with PLS-DA to quantify elements found in human gallstones.
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Hyperspectral Imaging Combined with Convolutional Neural Network for Rapid and Accurate Evaluation of Tilapia Fillet FreshnessThe purpose of this work is to achieve rapid and nondestructive determination of tilapia fillets storage time associated with its freshness. Here, we investigated the potential of hyperspectral imaging (HSI) combined with a convolutional neural network (CNN) in the visible and near-infrared region (vis-NIR or VNIR, 397−1003 nm) and the shortwave near-infrared region (SWNIR or SWIR, 935−1720 nm) for determining tilapia fillets freshness.
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Single-Cell Analysis by ICP-MS—Current Status and Future TrendsSingle-cell analysis is important in biology and medicine, because it takes into account cell heterogeneity and cellular dynamics, which are governed by cellular crosstalk and the vicinity of cells. Thus, it is of utmost importance to obtain not only information about the heterogeneity of a cell population, but also about their spatial arrangement.
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A MATLAB Application for Calculation of Cell Pathlength in Absorption Transmission Infrared SpectroscopyReliable quantitative FT-IR measurements require that the pathlength be known to within 1%. Pathlength estimations based on nominal spacer thickness are not reliable and require that the actual pathlength be measured for accurate data. We demonstrate how.
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Raman Spectroscopy in Analyzing Fats and Oils in FoodsSeveral types of Raman spectroscopy, including Fourier transform (FT)–Raman and dispersive Raman, are well suited to examine and understand the fat compositional heterogeneity in solid foods, identify polymorph or crystallinity, and measure fatty acid saturation.
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A Dual Nanostructured Approach to SERS Substrates Amenable to Large-Scale ProductionSERS can amplify Raman signals, but to make the technique practical for industrial use, large quantities of substrate are needed. The approach described here could enable cost-effective, reproducible manufacturing of SERS substrates at large scale.
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Exploring the Potential of the Yb(III) (HE)4 Complex for Oncotherapy Using UV-vis SpectroscopyEvaluation of the UV-vis spectra of the reaction product of ytterbium (III) with hematoxylin (HE) indicates the formation of a rare earth complex that further reacts with marine mammal DNA, indicating the potential that this complex may have anti-tumor properties.
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Analysis of Elements in Snack Foods: A Closer Look at Pepperoni, Rice Noodles, Frozen Dinners, and PizzaSoftware tools for ICP-MS and ICP-OES can help analysts to simplify method setup and reduce the potential for errors.
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Detection of the Early Fungal Infection of Citrus by Fourier Transform Near-Infrared SpectraWe examine the feasibility of FT-NIR for the detection of early fungal infections in citrus.
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A Raman Spectral Area Scanning Method to Identify the Sequences of Crossed Writings and Seal StampsDetermining the printing sequences of crossed writings and seal stamps is often difficult because the most common methods used are expensive, time-consuming, and cumbersome. A new method using Raman spectral area scanning offers a better alternative while conducting pigment analysis and determining intersection sequences of writings and seal stamps. We explain why.
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Analyzing Water Samples Using EPA Method 200.8 to Support the Lead and Copper Rule RevisionsEPA Method 200.8 and the Lead and Copper Rule Revisions don’t allow use of modern ICP-MS technology with a collision cell. Instead, correction equations can be used to compensate for polyatomic interferences.
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Chemical Vapor Generation Non-Dispersive Atomic Fluorescence Spectrometry Technique for the Determination of Arsenic, Antimony, Selenium, and Mercury in Geological Samples by One-Time DigestionIn this paper, a one-time digestion method for the determination of arsenic (As), antimony (Sb), selenium (Se), and mercury (Hg) in geological samples was established.
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Colorimetric Determination of Tea-Stained Seashells Using the B&W Tek Quest X UV-vis-NIR SpectrometerThis study compares the sensitivity of CIE Lab values, peak area, and yellowness index for the determination of color attributes among a set of white and stained seashells exposed to tea tannins.
