Author | Jenni L. Briggs

Jenni Briggs is with Aspectrics, Pleasanton, California.

Articles

Simple Method for Monitoring Protein Secondary Structure During Thermal Unfolding and Aggregation

April 01, 2016

Spectroscopy

Article

Protein secondary structure during thermal unfolding and aggregation is readily acquired using IR spectroscopy and a temperature-controlled mid-IR transmission accessory. Myoglobin was used as a model system to illustrate the method.

Simple Method for Monitoring Protein Secondary Structure during Thermal Unfolding and Aggregation

March 16, 2016

Spectroscopy

Article

Protein secondary structure during thermal unfolding and aggregation is readily acquired using IR spectroscopy and a temperature-controlled Mid-IR transmission accessory.

Simple Method for Monitoring Protein Secondary Structure During Thermal Unfolding and Aggregation

February 01, 2016

Application Notebook

Article

As the temperature increases, significant changes in the myoglobin secondary structure are observed. Between 25 °C and 70 °C, the 1649 cm-1 peak height decreases and linewidth increases. These changes in the amide I band reflect increased protein structural disorder. A temperature increase from 70 °C to 75 °C causes a drop in the 1649 cm-1 band intensity. Simultaneously, two peaks grow in at 1683 and 1637 cm-1. These changes indicate conversion of random coil/α-helix to intermolecular β-sheet. Further conversion to β-sheet is observed as the temperature rises to 90 °C. The final β-sheet structure is an insoluble aggregate. This aggregation process is not reversed when the temperature is driven back to 25 °C.

Infrared Transmission Measurements of Diffuse Samples

September 01, 2015

Application Notebook

Article

Measuring the transmission of diffuse samples such as paper or filled polymer films requires an integrating sphere to obtain accurate transmission values and useful chemical information, because the IR light is scattered as it passes through the sample. An integrating sphere serves as a collection device for the scattered light.