February 01, 2022
Using an automated FT-IR reflection sampling accessory, the surface of a polymer-coated aluminum film was analyzed. Spectral results were used to investigate chemical uniformity across the sample area.
September 01, 2021
June 04, 2021
Data show that for applications where increased absorbance leads to added sensitivity, a variable angle ATR offers a powerful tool for making FTIR measurements.
September 01, 2018
November 10, 2016
Ocean microbiological activity of chemical species present in the sea surface microlayer was investigated using ATR-FTIR spectroscopy. We observe chemical differences before and during the peak of an induced phytoplankton bloom.
March 16, 2016
Protein secondary structure during thermal unfolding and aggregation is readily acquired using IR spectroscopy and a temperature-controlled Mid-IR transmission accessory.
February 01, 2016
As the temperature increases, significant changes in the myoglobin secondary structure are observed. Between 25 °C and 70 °C, the 1649 cm-1 peak height decreases and linewidth increases. These changes in the amide I band reflect increased protein structural disorder. A temperature increase from 70 °C to 75 °C causes a drop in the 1649 cm-1 band intensity. Simultaneously, two peaks grow in at 1683 and 1637 cm-1. These changes indicate conversion of random coil/α-helix to intermolecular β-sheet. Further conversion to β-sheet is observed as the temperature rises to 90 °C. The final β-sheet structure is an insoluble aggregate. This aggregation process is not reversed when the temperature is driven back to 25 °C.
November 03, 2015
An infrared transmission spectrum of lens tissue was collected and compared with a sample spectrum collected in the FT-IR sample compartment. Benefits of using an integrating sphere are discussed.
September 01, 2015
Measuring the transmission of diffuse samples such as paper or filled polymer films requires an integrating sphere to obtain accurate transmission values and useful chemical information, because the IR light is scattered as it passes through the sample. An integrating sphere serves as a collection device for the scattered light.
September 01, 2014
The thermal treatment of a grafted Sn-SiO2 catalyst was monitored in situ using diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS).