Biopharmaceutical Analysis with Submicron IR+Raman Spectroscopy

Submicron optical photothermal infrared (O-PTIR) spectroscopy is transforming particulate and aggregate analysis in biopharmaceuticals by delivering true IR and Raman chemical specificity below the diffraction limit. This paper explores how O-PTIR overcomes long-standing challenges associated with FTIR, Raman, and fluorescence techniques, enabling high-resolution, artefact-free characterization of complex protein and polymeric particulates. Real-world case studies demonstrate how simultaneous IR+Raman measurements reveal chemical heterogeneity and insights critical for reducing immunogenicity in advanced therapeutics.