	Historically, ion-exchange chromatography and size-exclusion chromatography (SEC) have been the reference techniques for the physicochemical characterization of biopharmaceutical compounds. However, reversed-phase liquid chromatography is naturally more compatible with mass spectrometry detection. This article discusses new trends in the use of ion-exchange chromatography and SEC, as well as the power of using ultrahigh-pressure liquid chromatography (UHPLC) and advanced reversed-phase LC columns, including sub-2-µm wide-pore porous particles or core–shell particles, for biopharmaceutical characterization.	 	Read more 

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Articles in this Issue

Analytical Tools for the Characterization of Biopharmaceuticals: Chromatographic Methods

	A complete set of mass spectrometry tools is available for mAb characterization, and includes bottom-up approaches, middle-up analysis of mAb fragments, and intact mAb analysis. In some cases, these tools are being coupled with chromatographic separation methods while in others they are being used with innovative techniques such as native MS and native ion mobility mass analysis. In particular, native MS techniques can play a key role in the characterization of higher-order structures.	 	Read more 

Analytical Tools for the Characterization of Biopharmaceuticals: Mass Spectrometric Detection

	A very important component of the characterization of therapeutic proteins is the analysis of the carbohydrate structures that are attached to them. Those carbohydrates play an important role in ensuring the safety and efficacy of these drugs, and are an important consideration when comparing a candidate biosimilar drug to its reference product. For advice on this topic, 

 spoke to Parastoo Azadi, PhD, the technical director for analytical services at the University of Georgia Complex Carbohydrate Research Center.	 	Read more 

Spectroscopy E-Books-11-01-2014

Analytical Tools for the Characterization of Biopharmaceuticals: Chromatographic Methods

Analytical Tools for the Characterization of Biopharmaceuticals: Mass Spectrometric Detection

Analyzing Carbohydrates on Therapeutic Glycoproteins