June 6th 2024
Scientists from the Czech Republic recently created new practices to study different Tröger’s base isomers.
A Novel Approach to Measure Crop Plant Protein Expression
July 1st 2012Liquid chromatography–mass spectrometry (LC–MS) successfully differentiated transgenic from native protein in a case where the proteins were highly homologous and could not be differentiated by traditional methods. This methodology may be useful for other studies of transgenic crops.
Reliable and Efficient Sulfur Detection in Proteins Using ICP-MS with Capillary LC
October 1st 2010With recent research, the University of Oviedo's analytical spectrometry research group has taken a step closer to the absolute quantification of proteins. Quantification based upon isotope dilution mass spectrometry of sulfur is hampered by gas-based polyatomic interferences. By implementing a quadrupole inductively coupled mass spectrometer with collision/reaction cell technology, the group has been able to overcome the issues and has increased reliability while optimizing the efficiency of its analyses.
Protein Identification in Complex Mixtures: A Comparison of Accurate-Mass Q-TOF and Ion-Trap LC–MS
March 1st 2008Because it is extremely rapid, biomarker discovery and identification using liquid chromatography–mass spectrometry (LC-MS), including both ion-trap and triple-quadrupole LC–MS, is well established. Fractionation of complex samples before LC–MS-MS analysis might be necessary to identify the proteins, greatly increasing the number of analyses required. In this case, there is ongoing debate regarding knowing whether the protein is identified correctly, knowing how much prior fractionation is needed to reduce complexity to the point where low-abundance proteins can be detected reliably, and balancing specificity with sensitivity.