Spectroscopy Interviews

Geochronology is an exciting area of atomic spectroscopy and earth science research. One of the goals is to answer tectonic questions, and in particular, how the crust responds to continent–continent collision. John M. Cottle, a professor of earth science at the University of California, Santa Barbara, is one of the scientists on that mission. Cottle and his research group are at the forefront of discovery in geochronology, combining both laboratory and field-based research. In particular, Cottle is a leader in the development of novel laser-ablation inductively coupled plasma–mass spectrometry (LA-ICP-MS) measurements and their application to tectonic questions in convergent orogens, which are mountain ranges formed when a continental plate crumples and is pushed upwards.

Near-infrared (NIR) spectroscopy is an important technique in the pharmaceutical industry because of its ability to provide information about bulk material without sample preparation. Multivariate calibration techniques are frequently used to analyze the NIR data. Robert Lodder, who is a professor in the Department of Pharmaceutical Sciences at the University of Kentucky in Lexington, Kentucky, uses NIR spectroscopy along with an interesting alternative calibration technique, molecular factor computing, in his work with an experimental drug for combating the Ebola virus. We recently spoke with him about his research.

The atomic spectroscopy techniques of laser-induced breakdown spectroscopy (LIBS) and X-ray fluorescence spectroscopy have different strengths. Lydia Breckenridge, a senior research investigator at Bristol-Myers Squibb, uses both techniques in her work in pharmaceutical development. Here, she shares some of the advantages and challenges of using these techniques, and how the greatest benefits are sometimes derived by focusing on their complementarity, and using them in combination.

Two-dimensional (2D) Raman correlation spectroscopy is a powerful analytical technique for analyzing a system under the influence of an external perturbation. Isao Noda, of the Department of Materials Science and Engineering, at the University of Delaware and Danimer Scientific, has been developing 2D Raman correlation spectroscopy and applying it to the study of various materials, including exciting new biopolymers. He recently spoke to us about this work.

In recent years, researchers have been making important developments to advance the effectiveness of spectroscopic techniques for biomedical uses ranging from the identification of infectious agents to measuring the edges of cancerous tumors. X-ray fluorescence (XRF) spectroscopy is among the techniques that can have useful medical applications. David R. Chettle, a professor in the Department of Physics and Astronomy at McMaster University in Hamilton, Ontario, Canada, uses XRF for the in vivo measurement of toxic elements in human subjects, with the goal of developing devices that can be used to investigate the possible health effects of toxin exposure. He recently spoke to us about his research.

Bioanalysis, and particularly medical diagnostics, is an exciting area of spectroscopy research. One of the dreams is to develop spectroscopic tools that can be used for point-of-care diagnostics with a smartphone. Russ Algar, an assistant professor in chemistry at the University of British Columbia (UBC), in Vancouver, Canada, is one of the scientists on that quest. Algar and his research group focus on the development of nontraditional fluorescent materials-such as quantum dots, luminescent lanthanide complexes, and semiconducting polymer dots-for biochemical sensing. They are studying how these materials can be applied to a variety of problems, including molecular medicine, personalized medicine, and yes, point-of-care diagnostics with smartphones. For his work, Algar has been chosen as the winner of second annual Emerging Leader in Molecular Spectroscopy award, presented by Spectroscopy magazine.

In recent years, there have been significant advances in the application of vibrational spectroscopy to the analysis of forensic samples. Igor K. Lednev, a professor in the Department of Chemistry at the University at Albany, the State University of New York, has been developing the use of Raman spectroscopy for a variety of forensic applications, including the determining the age of blood stains and linking gunshot residues to specific ammunition–firearm combinations. He recently spoke to Spectroscopy about his work.

There is growing concern about the unknown effects that nanoparticles may have on the environment, especially in drinking water and plants. Single-particle inductively coupled plasma–mass spectrometry (SP-ICP-MS) is emerging as a useful technique for analyzing nanoparticles and their presence in environmental and biological systems. Honglan Shi, a chemistry professor at Missouri University of Science and Technology, and her research group have been using SP-ICP-MS to investigate nanoparticles in drinking water and plant uptake. She recently spoke to Spectroscopy about this work.

In forensic science, the detection of blood on fabric is a very useful tool. Therefore, it is important that the methods used for detecting blood be as accurate as possible. Michael L. Myrick and Stephen L. Morgan, both professors in the Department of Chemistry and Biochemistry at the University of South Carolina, have been investigating the use of infrared (IR) spectroscopy for this purpose, including comparing the effectiveness of infrared diffuse reflectance versus attenuated total reflectance Fourier-transform IR (ATR FT-IR). They recently spoke to Spectroscopy about their recent studies and the critical questions they have been addressing in how IR spectroscopy is used in forensic science.

Although laser-induced breakdown spectroscopy (LIBS) potentially can be used for practically any kind of sample, most applications have focused on solid sample analysis. Montserrat Hidalgo, a professor in the Department of Analytical Chemistry and Food Sciences and the University Institute of Materials at the University of Alicante in Alicante, Spain, has been working with various approaches to extend the applicability of LIBS to trace-elemental analysis of liquid samples. She recently spoke to us about this research.

Naoto Nagai, of the Industrial Research Institute of Niigata Prefecture in Japan, has been studying the potential of IR spectroscopy for investigating higher-order structures of polymers. He and his colleagues recently looked at the IR spectra of polyoxymethylene (POM) mold plates and the cause of occasional resin cracks.

In drug development, quantitative determination of a candidate drug and its metabolites in biofluids is an important step. The standard technique for quantitative metabolite profiling is radiolabeling followed by high performance liquid chromatography (HPLC) with radiodetection, but there are disadvantages to this approach, including cost and time, as well as safety and ethical concerns related to administering radiolabeled compounds to humans.

Surface-enhanced Raman spectroscopy (SERS) with silver nanorod-array substrates has been used in various biological applications, such as detection of proteins in body fluids. Duncan C. Krause, who is a professor in the Department of Microbiology at the University of Georgia, worked with his group to establish a SERS method with those substrates for detecting the pathenogenic mycoplasma that causes bronchitis and pneumonia. We recently spoke with him about this research.

Coherent two-dimensional infrared spectroscopy (2D IR) uses a series of IR femtosecond laser pulses to pump and then probe the response of a system, making it possible to learn much more about the structure and dynamics of molecules than can be seen with one-dimensional IR spectroscopy. The technique’s inventor, Martin T. Zanni of the University of Wisconsin-Madison, discussed 2D IR in a 2013 interview in Spectroscopy (1). Since 2013, Zanni has applied 2D IR spectroscopy to new systems and has started a company, PhaseTech Spectroscopy, Inc., to commercialize the technique.

Proteomics and structural biology require specialized mass spectrometry methods for characterizing protein structures and conformations. Jennifer S. Brodbelt, a professor of chemistry at the University of Texas at Austin, focuses on the development and application of photodissociation mass spectrometry for studying biological molecules such as peptides, proteins, nucleic acids, oligosaccharides, and lipids. She recently spoke with Spectroscopy about her work with this technique. She is the winner of the 2017 ANACHEM Award, which will be presented at the SciX meeting in October 2017. The award is presented annually to an outstanding analytical chemist based on activities in teaching, research, administration, or other activities that have advanced the art and science of the field.

Using Raman and FT-IR spectroscopy for on-line monitoring of manufacturing processes offers advantages such as improved quality control, nondestructive analysis, and reduced costs. Jim Rydzak has more than 20 years of experience leading teams in applying on-line process control, in both the pharmaceutical and consumer goods industries. He recently talked to Spectroscopy about that work, including what they achieved and how they overcame challenges.

Multiple-collector inductively coupled plasma–mass spectrometry (MC-ICP-MS) is a powerful technique for measuring isotopic ratios in various areas of research. Michael Wieser, who is an associate professor in the Department of Physics and Astronomy at the University of Calgary, uses MC-ICP-MS to measure isotopic compositions at trace levels in applications ranging from geological studies to protein research. He recently spoke to Spectroscopy about this work.

In biomedical applications of surface-enhanced Raman spectroscopy (SERS), nanoparticles can enhance the Raman signal and provide additional functionality. Duncan Graham of the University of Strathclyde has been pushing the limits of what can be achieved using functionalized nanoparticles and SERS, in applications such as cholera detection, lipid profiling in cancer cells, and assessing the efficacy of anti-cancer drugs, For this and other work he has won the 2017 Charles Mann Award, presented by the Federation of Analytical Chemistry and Spectroscopy Societies (FACSS). He recently spoke to Spectroscopy about this work. This interview is part of a series of interviews with the winners of awards that will be presented at the SciX 2017 conference in October.

Spectroscopy is proud to have created a new award, the Emerging Leader in Atomic Spectroscopy Award. As its name implies, the award recognizes a young scientist, and it is designed to encourage the next generation of atomic spectroscopists. George Chan, the winner of the inaugural Emerging Leader in Atomic Spectroscopy Award, is a project scientist at Lawrence Berkeley National Laboratory (LBNL) in Berkeley, California. One of Chan’s most significant contributions to the field of atomic spectroscopy has been his work on matrix effects and excitation processes in the inductively coupled plasma (ICP).

Optical coherence tomography (OCT) is an emerging technique for medical imaging that uses light to see deep inside tissue. Rohith Reddy, who is a postdoctoral research fellow at the Harvard Medical School and Massachusetts General Hospital in Boston, has worked to develop an OCT device for noninvasive diagnosis of a precancerous condition, Barrett’s esophagus. Reddy is the winner of the FACSS 2016 Innovation Award. He recently spoke to us about these efforts.

Surface-enhanced Raman scattering (SERS) has the capability of enhancing the signal from analytes present in low concentrations, and the detection of drugs present in human and other samples is an important application of this technique. Roy Goodacre is a Professor of Biological Chemistry in the School of Chemistry at the University of Manchester, and he and his group have been developing SERS methods for analyzing drugs in various solutions, including human biofluids, with the ultimate goal of monitoring dosing and drug therapy. He recently spoke to us about this work.

Metals and metalloids, while essential to living organisms, can, in high concentrations, be toxic. An understanding of how these metals and metalloids are accumulated and transported within plants and animals is possible with the use of synchrotron X-ray fluorescence (SXRF) microtomography. The technique is used in the imaging of major and trace element distributions within natural materials with high spatial resolution. Stefan Vogt of the Argonne National Laboratory in Lemont, Illinois, has been exploring the use of SXRF to detect metal content in biological and other systems. He recently discussed the various challenges, applications, and advantages associated with this technique.

Isolating material of commercial value from solid natural products presents a challenge for many spectroscopic techniques. Near-infrared (NIR) chemical imaging makes it possible to obtain spectra from individual pixels within a field of view for analysis of complex, heterogeneous mixtures. A team at Kansas State University, led by David Wetzel, has been applying this approach to multiple applications, including the analysis of wheat. In particular, the group has work on alternative methods for the determination of flour and milling stream purity, because outdated methods such as mineral ash residue impurity analysis do not properly reflect the quality of the final products of milling and are dependent upon the soil where the wheat is milled. Mark Boatwright, who is studying for his doctorate under Wetzel, talked to Spectroscopy about some of this work.

The analysis of metals using inductively coupled plasma–mass spectrometry (ICP-MS), ICP-atomic emission spectroscopy (ICP-AES), and atomic absorption can serve many purposes in environmental, health, and forensic studies. Yi He, a chemistry professor at John Jay College of Criminal Justice at The City University of New York, has been using these elemental analysis techniques for fingerprinting and provenance of counterfeit cigarettes and as an educational tool. Here, she discusses some of that work.

Spectroscopy is proud to have created a new award, the Emerging Leader in Molecular Spectroscopy Award. As its name implies, the award recognizes a young scientist, and it is designed to encourage the next generation of molecular spectroscopists. Matthew Baker, the winner of the inaugural Emerging Leader in Molecular Spectroscopy Award, is a senior lecturer in chemistry at the University of Strathclyde, in Glasgow, Scotland. At Strathclyde, Baker leads research to advance the application of analytical chemistry to real-world problems in a variety of areas, including the biomedical, clinical, defense, and security fields. His main focus is the development of spectroscopic and spectrometric molecular pathology, disease diagnosis, and the detection of pathogenic bacteria and toxic chemicals. In particular, Baker has pioneered the use of vibrational spectroscopy for clinical diagnostics.

Advances in spatial resolution for Fourier transform infrared (FT-IR) imaging historically have involved the use of a synchrotron source, but new optics have been developed that yield better spectral quality and spatial resolution than are provided by existing synchrotron sources. Kathleen Gough, Professor in the Department of Chemistry at the University of Manitoba, has been working with her group to conduct diagnostic tissue imaging with the new thermal source FT-IR system. She recently spoke to us about these efforts.

The use of engineered nanoparticles (ENPs) in various applications and consumer products continues to increase, and these nanoparticles require thorough characterization for proper environmental risk assessment. James Ranville, a professor at the Colorado School of Mines, in Golden, Colorado, has been studying colloids and and particles in environmental processes and developing methods to collect and analyze colloids from rivers, reservoirs, mountain streams, soil solutions, and ground waters. He spoke with us about his work using field-flow fractionation–inductively coupled plasma mass spectrometry (FFF-ICP-MS) and ICP-MS for the detection of engineered nanoparticles in environmental samples.

Currently, there is significant interest in using vibrational spectroscopy techniques for a variety of biomedical applications, and the methods are showing good promise. Karen Faulds, a professor at the University of Strathclyde in Glasgow, has been investigating the application of surface-enhanced Raman spectroscopy (SERS) to the detection of disease pathogens, such as meningitis, and to distinguish related pathogens in a complex matrix. Faulds is the 2016 recipient of the Coblentz Society’s Craver Award, which recognizes the efforts of young professional spectroscopists who have made significant contributions in applied analytical vibrational spectroscopy. This interview is part of a series of interviews with the winners of awards that will be presented at the SciX 2016 conference.

Laser-ablation inductively coupled plasma–mass spectrometry (LA-ICP-MS) is well suited for highly sensitive elemental and isotopic analysis of solid samples. In this technique, a laser beam ablates the sample and generates fine particles that are then transported to the ICP-MS system for rapid elemental analysis. Detlef Günther is Professor for Trace Element and Micro Analysis and Vice President Research and Corporate Relations for ETH Zurich, and he and his group use LA-ICP-MS for two- and three-dimensional imaging of geological samples such as rocks and meteorites. He recently spoke to us about this research.

Spectroscopy has played a significant role in the Mars expeditions, including the confirmation of the former presence of water on the Red Planet. Raymond Arvidson, the James S. McDonnell Distinguished University Professor at Washington University in Saint Louis, Missouri, is involved with the various National Aeronautics and Space Administration (NASA) missions to Mars and the spectroscopy incorporated in the instruments sent there. Here, Arvidson discusses those techniques, including a hyperspectral imaging system, an emission spectrometer, and an X-ray spectrometer, and what the results of the missions indicate about Mars so far.