SPEC PerkinElmer 10.13
|Articles|April 15, 2017

Thermal Denaturation of DNA Using UV–Vis

Author(s)PerkinElmer

UV/Visible Spectrophotometry can be used to monitor the thermal denaturation of DNA as the sample is heated allowing determination of the DNA melt temperature, tm, and ultimately the %G-C content.

 


Related to this article

Infrared Reimagined: How FT-IR Spectroscopy Learned to Read Molecules, ©  Luis Eduardo  -chronicles-stock.adobe.com
FT-IR spectroscopy, long treated as a mature bench technique for confirming a carbonyl stretch or fingerprinting a polymer, has quietly become one of chemistry’s most versatile discovery engines: it now infers molecular structure straight from a spectrum without a reference library, resolves chemistry tens of nanometers wide, and screens a fingerstick of blood for disease in minutes. The last five years of published research show FT-IR moving from a confirmatory tool into a predictive, autonomous, and field-ready analytical platform.
The Ongoing Decline of Spectroscopy Expertise
In this panel discussion, five SpecAcademy instructors examined why spectroscopy expertise has declined and how SpecAcademy aims to rebuild it through curated, fundamentals-based education suited to increasingly complex analytical work.