Application Notes: General

The Cary 3500 UV-Vis systems use temperature-controlled UV-Vis spectroscopy to analyze molecules' thermal stability. They are essential for nucleic acid quantification quality control by measuring absorbance at 260 nm and performing thermal melt identification verification. Melting temperature (Tm) analysis is key for studying nucleic acid denaturation.

All starting materials, including APIs, must undergo analytical identity (ID) determination before pharmaceutical manufacturing to ensure drug safety. Oligonucleotides pose challenges for ID due to sequence specificity. Melting temperatures (Tm) combined with intact mass measurements offer a solution using UV-based spectrometry for precise Tm determination at Vetter Pharma.

Vetter Pharma successfully uses the Cary 3500 UV-Vis to optimize thermal melt measurements of oligonucleotide-based pharmaceuticals.

Characterizing biomolecules such as oligonucleotides and proteins is critical to better understanding biochemical and molecular processes. UV-Vis spectroscopy is widely used for stability studies, protein aggregation, DNA quantification and purity, and enzyme activity. The Cary 3500 UV-Vis offers advanced features to provide accurate and reproducible results.

The drug discovery process involves identifying and characterizing drug targets to find lead compounds. Biochemical characterization (e.g., molecular weight, solubility, thermal stability) is crucial for protein therapeutics. Thermal stability studies measuring melting temperatures help understand protein stability, ligand-binding, and optimal storage, aiding drug development and commercialization.

This application note demonstrates the performance of a Milli-Q® ultrapure water system for the quality control of metals in food and water samples in regulated labs.

This application note demonstrates the performance of a Milli-Q® ultrapure water system for the quality control of metals in food and water samples in regulated labs.

The pharmaceutical industry relies on Raman microscopy for product analysis and quality control. This technique is crucial for investigating a vast range of products, from life-saving medicines to everyday over-the-counter treatments.

Olive oil, especially extra virgin, is prized for its quality and purity. However, fraud and adulteration are significant concerns. UV-Vis spectrophotometry is a reliable technique for assessing olive oil quality by quantifying key compounds. An Edinburgh Instruments DS5 Spectrophotometer is used to evaluate olive oil quality and detect adulteration.

Photon upconversion converts low-energy photons to higher-energy photons. UCNPs, composed of rare-earth elements, can convert near-infrared light to visible or UV light. They have potential applications in solar energy and biomedicine. Understanding their luminescence properties is crucial for optimization. FS5 Spectrofluorometer was used for comprehensive upconversion characterization.

Raman OEM applications need consistent spectra to deliver dependable answers. Learn how to achieve >99.5% unit-to-unit agreement using a series of simple corrections.

Portable Raman spectroscopy bridges the gap between handheld & benchtop Raman. Learn the factors to consider when choosing a spectrometer, for research or OEM use.

Photosensitisers (PS) absorb light and reach a triplet excited state. This state interacts with a target species, transferring energy and inducing reactions. PS returns to its ground state and can participate in further photochemical activations.

GaN, a wide-bandgap semiconductor, surpasses silicon in high-voltage and high-temperature applications. It's crucial for energy-efficient LED lighting and laser technology, revolutionizing the industry.

Light is utilized in a wide range of spectroscopic techniques and practices to gain a variety of information about materials and chemicals. These analyses can include material/chemical identification, quantitative analysis, quality control/quality assurance testing, and biological studies, among other applications. Applications, along with sample handling for both FTIR and UV-Vis spectroscopy, will be outlined in this paper.

Ensure medical device safety and compliance with our advanced testing solutions. Discover technologies that enhance reliability, meet regulatory standards, and improve patient outcomes. Download our brochure to support your device development and compliance journey.

The PerkinElmer Spectrum Two FTIR spectrometer and IR biodiesel application pack enable precise FAME biodiesel analysis in diesel blends per EN14078 standards, offering a simple workflow, 0–50% calibration range, and compliance assurance for QC labs.

Using the LAMBDA 1050+ UV/Vis/NIR Spectrophotometer and ASTM E 903-20, this study measured Total Solar Reflectance (TSR) of paints. White had the highest TSR, dark green the lowest, optimizing materials for energy-efficient applications.

Discover how the PerkinElmer FL 6500 fluorescence spectrophotometer enhances Euro banknote security by detecting fluorescent features with unmatched precision. Ideal for forensics and law enforcement, it ensures currency integrity and combats counterfeiting effectively.

Vaccines save millions annually, yet their journey from lab to clinic demands rigorous testing. Our brochure explores cutting-edge analytical solutions driving vaccine development, from upstream analysis to fill-and-finish, ensuring readiness against ever-evolving pathogens.

Using the PerkinElmer LAMBDA® 365+ UV/Vis spectrophotometer, this application note demonstrates RNA quantification via Beer-Lambert’s Law, constructing calibration curves and assessing RNA purity through absorbance ratios at 260 nm for precise analysis.

This application note highlights the PerkinElmer OilExpress 4 Platform for automated FAME biodiesel analysis, combining FTIR spectroscopy with high-throughput efficiency, processing up to 384 samples, ensuring accurate fuel quality testing while simplifying workflows in busy labs.

Discover the PerkinElmer LAMBDA 365+ UV/Vis spectrophotometer with a 6° fixed angle specular reflectance accessory, enabling precise material characterization for solar, semiconductor, and optics industries, enhancing quality control with easy alignment and operation.

Explore advanced hyphenated techniques like TG-IR and TG-GC/MS in this applications compendium, showcasing innovative solutions for materials analysis, energy storage, and environmental monitoring. Unlock groundbreaking insights and revolutionize your laboratory’s analytical capabilities today.

PerkinElmer’s fluorometric assay, using the FL 6500 spectrometer, provides critical insights into enzyme kinetics. Studying horseradish peroxidase (HRP) with Michaelis-Menten modeling, it highlights substrate concentration's impact on reaction rates, aiding process optimization across industries.