Mass Spectrometry

A summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS

A discussion of active pharmaceutical ingredient (API) selection, drug product development, and mass spectrometry instrumentation

A review highlighting the combination, or potential combination, of various separation methods with ambient ionization mass spectrometry

Spectroscopy invites researchers to submit their work for publication.

A breakthrough using a microfluidic interface to conduct sensitive time-of-flight secondary ion mass spectrometry (TOF-SIMS) analysis and study liquid surfaces in situ under vacuum conditions is described here.

Consequences of Finite Ion Lifetimes in Mass Spectrometry As we construct more-complex instruments that process packets of ions in time and space, the issue of ion lifetimes is becoming more important.

High-definition screening by gas chromatography–mass spectrometry (GC–MS) is shown to be a viable option for the reliable identification of odorous compounds in pork.

Mass spectrometry is now among the tools used by first responders to identify potential threats, but there are challenges involved, including interpreting results and ensuring data relevance.

This study illustrates the versatility of a laser-induced acoustic desorption (LIAD) approach for volatilization of analytes in modern mass spectrometry.

The combination of GC with time-of-flight (TOF)-MS proves to be a successful approach for the challenging analysis of persistent organic pollutants in complex matrices such as sediment and fish samples.

More-specific, sensitive, rapid, and portable spectroscopic methods detect biological threats.

The revisions to GMP Annex 11 are examined in terms of the impact on computerized spectrometry systems operating in regulated GMP laboratories.

A fluoride-regeneration approach enables biomonitoring of chemical warfare nerve agents.

Increases in vitamin D testing frequency have required more rapid and cost-effective solutions for determining vitamin D levels in plasma. This LC–MS method was adapted for use with core-shell columns to achieve run times of less than 4 min.

Most plants used in traditional Chinese medicine must be processed before their medicinal usage; hence the effective ingredients may differ from those in the freshly harvested plant extracts. In this work, we present a fast and generic approach using sub-2-?m liquid chromatography–time-of-flight–mass spectrometry (sub-2-?m-LC–TOF-MS) coupled with multivariate statistical data analysis to systematically profile ingredient changes between fresh and processed samples of huang jing.

Fossilomics uses MS to extract amino acid sequence information from subpicomole quantities of protein and peptide fragments that remain in certain fossil samples. The sequences are compared to databases and validated with search statistics and high-confidence sequences. The validated sequences can then be used to place the fossils on the evolutionary tree.

The inclusion of time-resolved selected reaction monitoring (SRM) functionalities into mass spectrometer control software allows large numbers of peptides to be quantified using short LC–MS-MS methods.

This new data-management method is specifically designed to handle the large amounts of data produced in proteomics studies. Experimental methods and data are presented from a recent study conducting proteomics profiling of Schizosaccharomyces pombe to demonstrate the effectiveness of the data management solution. The results demonstrate enhanced data search speeds as well as effective data analysis.

The metabolomics workflow described here combines untargeted (discovery) quadrupole time-of-flight (Q-TOF) liquid chromatography–mass spectrometry (LC–MS), targeted (confirmation) triple-quadrupole LC–MS-MS, and sophisticated data mining as an effective means to elucidate metabolite changes.

Analyses performed with hybrid mass spectrometers yield large volumes of complex data at high speeds and resolution.

The spectroscopy market has benefited from the general return to global economic growth.

There are currently more than 1000 pesticides in use worldwide in the production of foodstuffs. There is a significant risk to human health and the environment due to increased pesticide use, poor agricultural practices and illegal use.

What tools can MS practitioners use to obtain unambiguous answers from unknown spectra?

This month's column describes the use of mass spectrometers in submarines and their more recent use for direct sampling of compounds dissolved in water at ever-increasing depths.

The analysis of urine for drugs of abuse via chromatographic methods is commonplace but can be complicated by high matrix effects and frequent coelution. Novel time-of-flight mass spectrometry in combination with sophisticated deconvolution software was tested and found to provide increased confidence in results due to the high sensitivity and quality of spectra achieved.