Mass Spectrometry

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Image Credit: © Oligo Factory
Synthesizing Synthetic Oligonucleotides: An Interview with the CEO of Oligo Factory

February 6th 2024

LCGC and Spectroscopy Editor Patrick Lavery spoke with Oligo Factory CEO Chris Boggess about the company’s recently attained compliance with Good Manufacturing Practice (GMP) International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) Expert Working Group (Q7) guidance and its distinction from Research Use Only (RUO) and International Organization for Standardization (ISO) 13485 designations.

Potassium. Kalium. Alkali metals. Chemical Element of Mendeleev's Periodic Table. Potassium in square cube creative concept. | Image Credit: © Aleksander - stock.adobe.com
Potassium Isotopic Composition in Plants Analyzed Using MC-ICP-MS

December 12th 2023

Diatoms, algae under microscopic view, phytoplankton, fossils, silica, golden yellow algae | Image Credit: © elif - stock.adobe.com
Detecting Cadmium in Marine Phytoplankton Using Single-Cell Inductively Coupled Plasma Mass Spectrometry

December 6th 2023

Laser beam light effect | Image Credit: © donatas1205 - stock.adobe.com
New Laser Techniques Unlock Deuterium Release from Aluminum Layers

November 24th 2023

Holistic medicine approach. Healthy food eating, dietary supplements, healing herbs and flowers. Turmeric, dried lavender, spirulina powder in wooden bowls, fresh berries, omega acid capsules | Image Credit: © jchizhe - stock.adobe.com
More Than Just Acronyms at EAS 2023

November 14th 2023

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The Role of Liquid Chromatography–Mass Spectrometry in the Characterization of Therapeutic Monoclonal Antibodies

May 1st 2016

Monoclonal antibodies (mAbs) have been increasingly used as biotherapeutic agents and a number of new mAbs are currently in the drug pipeline. Over the next five years the patent on at least nine major biotherapeutic monoclonal antibodies will expire, opening the door for development and marketing of generic forms known as Biosimilars. In this paper a review of the central role mass spectrometry coupled to liquid chromatography plays in characterizing these antibodies is presented. Contemporary top down and middle-up approaches using mass spectrometry and various novel separation techniques to measure the intact masses of mAbs and their subunits or domains are highlighted. Example data of an innovator mAb, Humira (adalimumab) are presented showing the identities and relative abundances of the isoforms associated with this mAb. Similarly the current state of classical peptide mapping using reversed-phase chromatography and tandem mass spectrometry with scan- dependent acquisition is briefly reviewed. Novel approaches that speed analysis and provide information on post translational modifications, glycosylation, and disulfide mapping are discussed. Example data of stressed and unstressed samples of adalimumab are also presented to demonstrate peptide mapping data and modifications to the antibody. Lastly, the current use of mass spectrometry in glycoprofiling of mAbs is reviewed. Example glycan data for adalimumab generated by a novel labeling scheme and sensitive to detection by both fluorescence and mass spectrometry will be presented.