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Top 10 Most Influential Biopharmaceutical  Articles on FT-IR © Emiliia -chronicles-stock.adobe.com

Fourier transform infrared (FT-IR) spectroscopy has undergone a notable evolution in biopharmaceutical analysis over the past three years. Advances in crystal engineering, process analytical technology (PAT), chemometrics, machine learning (ML), and hyphenated analytical platforms have significantly expanded FT-IR’s analytical scope. This article reviews ten of the most influential publications from 2023–2026 that exemplify FT-IR’s growing role across the biopharmaceutical lifecycle, from drug substance design and formulation to manufacturing, quality control, and clinical bioanalysis.

Top 10 Most Influential Biopharmaceutical Articles on Raman © Emiliia -chronicles-stock.adobe.com

Between 2023 and 2026, Raman spectroscopy transitioned from a supportive analytical technique to a central enabling technology in biopharmaceutical analysis and manufacturing. Advances in artificial intelligence (AI), machine learning (ML), automation, and surface-enhanced Raman spectroscopy (SERS) have expanded Raman’s role from nutrient monitoring to real-time prediction of critical quality attributes (CQAs), inline control of complex bioprocesses, and non-destructive analysis of finished drug products. This article reviews ten of the most influential publications from this period, highlighting how they collectively reshaped expectations for Raman spectroscopy as a process analytical technology (PAT) and a quality-by-design (QbD) tool in modern biopharmaceutical development.

This year’s Emerging Leader in Atomic Spectroscopy Award recipient is Sarah Theiner, whose research is focused on the application of atomic spectroscopy techniques—laser ablation inductively coupled plasma–mass spectrometry (LA-ICP-MS) and single-cell ICP-MS—to expand these analytical techniques as tools for biological and clinical imaging and drug-distribution studies.

A recent study investigated the use of laser-induced breakdown spectroscopy (LIBS) as a viable technique for detecting and quantifying trace technetium-99 (^99Tc) for future molten salt reactor applications, where long-lived fission products must be carefully monitored. Spectroscopy spoke to Hunter Andrews of Oak Ridge National Laboratory (Oak Ridge, Tennessee), corresponding author of the paper resulting from their research, about their findings.

Iron supplement tablets in woman’s hand for treating iron deficiency anemia. | Image Credit: agencies - stock.adobe.com

A study from the İzmir Institute of Technology published in Food Chemistry shows that combining FT-IR spectroscopy with chemometric modeling enables rapid, non-invasive screening of iron supplements and protein–iron complexes for their ability to restore cellular iron status in iron deficiency anemia models.

In our ongoing review of infrared spectra, we will study organic nitrogen containing compounds including amides and amines. Amides contain both nitrogen and a C=O group and are found in proteins and polymers. Amines contain carbon, nitrogen, and hydrogen, and are ubiquitous in medicines. As always, concepts will be illustrated with reference spectra.

San Antonio, Texas, USA | Image Credit: © SeanPavonePhoto - stock.adobe.com

Pittcon, one of the foremost laboratory science conferences, makes its Texas debut at San Antonio’s Henry B. González Event Center from March 7 to 11. The conference’s technical program of over 1100 sessions provides analytical scientists with direct access to the latest research and developments from an international assembly of top scientists and research pioneers.

Top 10 Most Influential Biomedical Articles on NIR © david-chronicles-stock.adobe.com

Over the past two years, near infrared spectroscopy (NIRS) and related NIR techniques have seen rapid adoption in biomedical research. These developments span non invasive diagnostics, functional monitoring, machine learning integration, point of care probes, and applications in complex clinical settings such as liver fibrosis, viral detection, neonatal care, brain injury, and neurodegenerative disorders. This article synthesizes 10 key publications, highlighting trends, methodologies, and clinical potential.

Most Influential Biomedical Articles on FT-IR © anima-chronicles-stock.adobe.com

Over the past three to four years, Fourier Transform Infrared (FT-IR) spectroscopy has emerged as one of the most rapidly expanding vibrational techniques in biomedical research. Driven by advances in attenuated total reflectance (ATR), live-cell measurements, chemometrics, and machine learning (ML), FT-IR has moved beyond descriptive biochemical profiling toward predictive diagnostics and translational clinical science. This article highlights and critically summarizes the top 10 most influential peer-reviewed articles published recently on FT-IR applications in tissues, cells, hair, blood, saliva, urine, and exercise physiology, emphasizing analytical innovation, clinical relevance, and future impact.