Application Notes

Surface Plasmon Resonance (SPR) is an optical technique that offers label-free biomolecular analyses, providing information on kinetic processes (association and dissociation), binding affinity, analyte concentration and real time molecule detection. It has become a powerful tool for the analysis of biomolecular events involved in drug development, cancer research, and antibody screening

Exposure to warm/humid conditions may cause pharmaceutical formulations to undergo change in hydration level, affecting properties and/or efficiency of the drug. Raman spectroscopy, with a temperature and humidity controller can mimic harsh conditions, monitor properties of ingredients and track modifications induced by environmental changes.

Time‐resolved Fluorescence can analyze potential health benefits from antioxidant activity of curcumin, found in turmeric. Curcuminoids, weakly fluorescent in aqueous solution, show quantum yield increases upon interaction with a protein, such as human serum albumin.

Locate particles, generate size/shape statistics, select particles based on size/shape, and acquire Raman spectra. Easily locate and identify particulate matter, including pharmaceutical materials, trace forensic evidence, geological rock/mineral particles, and airborne contaminants trapped on filters

Since the function of a protein is closely related to its structure, the structural analysis of proteins and peptides is becoming increasingly important to assist in deciphering bioactivity. CD measurements are easy to obtain, and small sample volumes can be used. Additionally, conformational changes of proteins and peptides due to pH, temperature and external ligands can also be obtained.

Alpha includes a teaching kit with comprehensive set of tools for universities and vocational schools to demonstrate and explain the application of FT-IR spectroscopy. Comes with starter package of different measurement accessories, wizard guided user interface and detailed instruction manual

The step-scan technique allows the monitoring of the temporal progress of very fast reproducible events (transients). The interferometer mirror consecutively steps to the separate interferogram points one by one where the experiment is restarted again

IR and Raman spectroscopy are powerful analytical techniques to detect, discriminate and identify material on a molecular level. Raman spectroscopy offers some advantages when applied to art and archaeology as well as earth sciences.

Advanced analytical methods and techniques for food control can help to safeguard human health and even lives. Bruker offers a comprehensive range of lab-based and in-situ solutions to detect and diagnose potential and existing threats and contaminations at any stage

Protein secondary structure during thermal unfolding and aggregation is readily acquired using IR spectroscopy and a temperature-controlled Mid-IR transmission accessory.

Patented UltraWAVE SRC technology goes beyond traditional digestion, offering greater capabilities for the most difficult sample types. High-performance stainless steel construction allows for higher pressures and temperatures, while disposable vessels eliminate the need to assemble, disassemble or clean between processing. Just as important, dissimilar samples can be processed simultaneously, saving time and money.

Up-conversion finds important applications from biological labels to sensors and solar cells. This application note elucidates the experimental requirements for the characterisation of up-conversion using lanthanides as a model material.

The combination of fast, modular spectrometers from Ocean Optics, with powerful chemometrics tools creates new opportunities for rapid, on-site testing of foods and other samples. From quick and inexpensive measurements in complex situations, to sample identification rivaling expert abilities or online quality control – the possibilities are endless.

Test new and used motor and hydraulic oils with the new liquid transmission analysis essential from Specac, the PearlTM.

The sensitive, selective, and real-time analysis characteristic of the SIFT-MS technique provides simple, robust, and continuous analysis of extremely diverse odor compounds at trace levels in air. This application note illustrates instantaneous, broad spectrum odor analysis with monitoring data from a chicken meat production facility.

This paper compares the performance of the Agilent OneNeb Series 2 nebulizer with conventional glass concentric and inert nebulizers recommended by PerkinElmer for use with the Optima Series ICP-OES systems.

Spectral Systems has developed its Diamond XP-BBAR™ coating. With our coating the SNR is about 30% better through most of the analysis region of a typical FT-IR instrument.

Spectral Systems is the world’s leading supplier of beamsplitter components our coatings can eliminate undesirable spectral features.

Spectral Systems’ Super-Sealed™ Liquid Cells are designed and manufactured for leak free precision quantitative analysis and maximum reproducibility.

Spectral Systems’ Infrared Windows are available in a wide variety of sizes, shapes and materials for measurement and operation in the infrared spectral domain.

A novel approach in Raman spectroscopy enables bulk material analysis without the need for specialized equipment. Enhanced Raman reflection can be integrated into current processes using available instrumentation, enabling real-time process measurement and control.

Closed vessel microwave digestion has proven to be an effective alternative to traditional sample prep techniques with methods of fast, complete digestions, clean environment, and full recovery of volatile compounds.

Explore how to determine amounts of different components in a mixture based on their relative thermal stability, by better separation, using a Thermogravimetric Analyzer.

Learn about temperature accuracy and obtaining reliable thermogravimetric data using improved temperature calibration and control algorithms in a Thermogravimetric Analyzer with accurate and responsive temperature control and read-out.

This applications note explores the potential of using a highthroughput single-reflection diamond ATR to detect structural changes in egg yolk upon heating.

As the temperature increases, significant changes in the myoglobin secondary structure are observed. Between 25 °C and 70 °C, the 1649 cm-1 peak height decreases and linewidth increases. These changes in the amide I band reflect increased protein structural disorder. A temperature increase from 70 °C to 75 °C causes a drop in the 1649 cm-1 band intensity. Simultaneously, two peaks grow in at 1683 and 1637 cm-1. These changes indicate conversion of random coil/α-helix to intermolecular β-sheet. Further conversion to β-sheet is observed as the temperature rises to 90 °C. The final β-sheet structure is an insoluble aggregate. This aggregation process is not reversed when the temperature is driven back to 25 °C.

Single reflection ATR spectroscopy is valuable for examining thermally induced changes in materials. Changes in the water concentration and amide bands of egg yolk were observed using the Harrick DiaMaxATR accessory. Further analysis is possible to obtain more rigorous identification of the heat-induced structural changes.

The flavonoids that dominate the fluorescence spectra for honey shown in Figure 1 are polyphenols. These plant metabolites determine the color, aroma, and flavor of the honey, and provide antioxidant and other health benefits. The fluorescence spectrum for each honey sample illustrates the sensitivity of fluorescence spectroscopy for characterizing honey.

The LUMOS FT-IR microscope is an all-in-one solution with an integrated spectrometer, a high degree of automation, and a dedicated user interface. The innovative motorized attenuated total reflectance (ATR) crystal allows performing the complete measurement procedure fully automated including background and sample measurements. The dedicated OPUS software Wizard guides the user through the whole measurement procedure. For identification of unknown compounds, spectrum search and mixture analysis are applied using the functionality of the OPUS/SEARCH package. Chemical images of the spectral data are generated from univariate and multivariate analysis results.

RISE microscopy, the combination of Raman imaging and scanning electron microscopy, is a powerful new analytical method for the analysis and interrelation of the structure and composition of microscopic samples. The integration of both imaging techniques in one instrument avoids shuttling the sample from one microscope to another. Here we demonstrated its usefulness in the identification, discrimination, and localization of polymorphs of titanium dioxide.